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AB308372

Anti-SARS CoV 2 COVID 19 nsp12 RdRP 抗体 [HL1444]

Anti-SARS CoV 2 COVID 19 nsp12 RdRP antibody [HL1444]

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(1 Publication)

Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line - SARS-CoV-2, Transfected cell lysate - SARS-CoV-2 samples. Cited in 1 publication.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp12 RdRP antibody [HL1444] (AB308372)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS CoV 2 COVID 19 nsp12 RdRP antibody [HL1444] (AB308372)

Immunohistochemical analysis of human SARS-CoV-2 (COVID-19) Nucleocapsid transfected HEK293T cell FFPE Cell Pellet Block labeling SARS CoV 2 COVID 19 nsp12 RdRP using ab308372 at 1/255 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp12 RdRP antibody [HL1444] (AB308372)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SARS CoV 2 COVID 19 nsp12 RdRP antibody [HL1444] (AB308372)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SARS CoV 2 COVID 19 nsp12 RdRP using ab308372 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL1444

アイソタイプ

IgG

キャリアフリー

Yes

交差種

SARS-CoV-2

アプリケーション

WB, ICC/IF, IHC-P

applications

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組合せ製品

Proteins & Peptides

AB283859

Recombinant Human coronavirus SARS-COV-2 Nsp7 protein

SDS-PAGE - Recombinant Human coronavirus SARS-COV-2 Nsp7 protein (AB283859)

  • 0
  • 0
Primary Antibodies

AB283958

Anti-SARS-COV-2 nsp3 antibody [EPR24842-84]

20ul selling size|RabMAb|Recombinant|Trial Size

ELISA - Anti-SARS-COV-2 nsp3 antibody [EPR24842-84] (AB283958)

  • 3
  • 3
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "SARS-CoV-2": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - SARS-CoV-2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/255", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - SARS-CoV-2": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SARS-CoV-2 nsp12 also known as nonstructural protein 12 functions as an RNA-dependent RNA polymerase (RdRp) important for viral RNA genome replication. This protein weighs approximately 106 kilodaltons and derives from the SARS-CoV-2 pathogen the virus responsible for COVID-19. Nsp12 integral to the replication complex is expressed primarily in the host cell's cytoplasm during coronavirus infection. Together with other nonstructural proteins it directs the replication and transcription of the viral genome thereby playing an important role in the viral life cycle.
Biological function summary

Nonstructural protein 12 associates with cofactors nsp7 and nsp8 to form a functional polymerase complex essential for replicating the viral RNA genome. This complex efficiently extends RNA strands from RNA templates using the 3' end as a starting point. Nsp12 contains a conserved polymerase domain which ensures the fidelity of replication. It performs extensive proofreading alongside overlapping proteins like nsp14 which provide exonuclease activity to correct errors sustaining the propagation of the virus within the host.

Pathways

The nsp12 protein directly interacts with mechanisms like viral RNA replication and viral mRNA synthesis. It holds a central position in the coronavirus replication-transcription complex working closely with other nonstructural proteins such as nsp7 and nsp8. These interactions enable the synthesis of full-length genomic RNA and shorter subgenomic RNAs critical for translating viral structural proteins therefore facilitating the continuous cycle of infection and propagation.

Nsp12's activity directly relates to COVID-19 pathogenesis making it a significant target for antiviral treatments. The inhibition of nsp12 through therapeutic agents can effectively disrupt viral RNA synthesis curbing the replication of SARS-CoV-2. Remdesivir a nucleotide analog drug that targets the RdRp activity of nsp12 exemplifies this strategy specifically impeding the SARS-CoV-2 replication process. Additionally variations in nsp12 and its associated protein interactions influence the severity and progression of coronavirus infections in affected individuals.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information SARS-CoV-2 PLpro
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:12633-12650 PubMed37955677

2023

KIFC1 promotes proliferation and pseudo-bipolar division of ESCC through the transportation of Aurora B kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Du,Lingyu Wei,Jia Wang,Yanyan Li,Jing Huo,Jinsheng Wang,Pu Wang
View all publications
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Abcam product promise

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