Anti-Sarcomeric Alpha Actinin 抗体 [EP2530Y] (ab68168)
![Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.co.jp/ps/products/68/ab68168/Images/ab68168-82379-anti-sarcomeric-alpha-actinin-antibody-ep2530y-western-blot.gif "Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2530Y] to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Sarcomeric Alpha Actinin antibody [EP2530Y]
Sarcomeric Alpha Actinin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2530Y] to Sarcomeric Alpha Actinin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Sarcomeric Alpha Actinin aa 750-850 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- Human skeletal muscle lysate and human muscle tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP2530Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68168の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/40.
WB: 1/20000 - 1/50000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: 1/40.
WB: 1/20000 - 1/50000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
Is unsuitable for Flow Cyt or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
組織特異性
Expressed in both skeletal and cardiac muscle. -
関連疾患
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
配列類似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
細胞内局在
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Entrez Gene: 291245 Rat
- Omim: 102573 Human
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
- Unigene: 498178 Human
- Unigene: 37638 Mouse
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別名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
画像
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Western blot - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168) at 1/50000 dilution + human skeletal muscle lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.co.jp/ps/products/68/ab68168/Images/ab68168-82378-anti-sarcomeric-alpha-actinin-antibody-ep2530y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)ab68168 at 1/100 dilution staining Sarcomeric Alpha Actinin in human muscles by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)](https://www.abcam.co.jp/ps/products/68/ab68168/Images/ab68168-3-benefits-of-recombinant-antibodies.png)
Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab68168 は 3 報の論文で使用されています。
- Gan L et al. A cell-penetrating peptide enhances delivery and efficacy of phosphorodiamidate morpholino oligomers in mdx mice. Mol Ther Nucleic Acids 30:17-27 (2022). PubMed: 36189424
- Xu J et al. Optimization of Timing and Times for Administration of Atorvastatin-Pretreated Mesenchymal Stem Cells in a Preclinical Model of Acute Myocardial Infarction. Stem Cells Transl Med 8:1068-1083 (2019). PubMed: 31245934
- Papadimitriou ID et al. A "human knockout" model to investigate the influence of the a-actinin-3 protein on exercise-induced mitochondrial adaptations. Sci Rep 9:12688 (2019). PubMed: 31481717