AB326352

Anti-SAP97 antibody [G28]

Recombinant
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Rabbit Recombinant Monoclonal SAP97 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse samples.

別名を表示する

Dlgh1, Dlg1, Disks large homolog 1, Synapse-associated protein 97, SAP-97, SAP97

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Neuroblastoma cells (Kelly). Species : Human. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SAP97 antibody [G28] (AB326352)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Pheochromocytoma cells (PC-12). Species : Rat. Fixation : 4% PFA for 10 min at RT. Permeabilization : 0.15% TritonX for 15 min at RT. Blocking : 10% goat serum for 40 min at RT. Primary Antibody : ab326352 at 1 : 100 for 1 hour at RT. Secondary Antibody : Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1 : 1000 for 1 hour at RT in the dark. Counterstain : DAPI (blue) nuclear stain at 1 : 1000 for 5 min at RT in the dark.

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Human SAP97 protein in HeLa whole cell lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 1 min at RT in the dark. Exposed 5 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 5s

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Rat SAP97 protein in a Rat brain membrane lysate. Block : 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 6.54 seconds.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Rat brain membrane lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 6.54s

Supplier Data

Western blot - Anti-SAP97 antibody [G28] (AB326352)

Western blot analysis showing detection of Mouse SAP97 protein in Mouse Brain lysate. Block : 5% BSA for 1 hour at RT. Primary Antibody : ab326352 at 1 : 1000 for 2 hours at RT. Secondary Antibody : Goat anti-rabbit IgG-HRP (H&L) at 1 : 5000 for 1 hour at RT. Color Development : Chemiluminescent for HRP for 3 min at RT in the dark. Exposed 1 second.

All lanes:

Western blot - Anti-SAP97 antibody [G28] (ab326352) at 1/1000 dilution

All lanes:

Mouse Brain lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP (H&L) at 1/5000 dilution

false

Exposure time: 1s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

G28

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human, Mouse

アプリケーション

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Promotes epithelial cell layer barrier function via maintaining cell-cell adhesion (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel (By similarity). During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane (By similarity).

See full target information Dlg1

追加のターゲット

DLG1_HUMAN,

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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