Anti-SAP97 抗体 [EPR26467-123] (BSA and Azide free)
Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free)
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal SAP97 antibody. Carrier free. Suitable for IP, IHC-Fr, IHC-P, WB and reacts with Mouse, Human, Rat samples.
別名を表示する
Disks large homolog 1, Synapse-associated protein 97, hDlg, SAP-97, SAP97, DLG1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAP97 with ab300481 at 1/2000 (0.234 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human colon (PMID : 7891172). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IP
Lab
Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. SAP97 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg (Inset) Lane 2 : ab300481 IP in SH-SY5Y whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300481 in SH-SY5Y whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds
All lanes:
Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/30 dilution
Lane 1:
SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate (Inset) at 10 µg
Lane 2:
<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a> IP in SH-SY5Y whole cell lysate
Lane 3:
Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 100 kDa
false
Exposure time: 5.5s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse liver (PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID : 7891172, PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SAP97 with ab300481 at 1/4000 (0.117 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on rat cerebrum (PMID : 7891172, PMID : 11181181). The section was incubated with ab300481 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
- IP
Lab
Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. SAP97 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate 10 µg (Inset) Lane 2 : ab300481 IP in Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300481 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds
All lanes:
Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/30 dilution
Lane 1:
Mouse brain tissue lysate (Inset) at 10 µg
Lane 2:
Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>)
Lane 3:
Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>)
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 100 kDa
false
Exposure time: 5.5s
- WB
Supplier Data
Western blot - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/1000 dilution
Lane 1:
HepG2 (human hepatocellar carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 20 µg
Lane 2:
HepG2 transfected with siRNAs specifically targeti DLG1, whole cell lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 130 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes:
Western blot - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/1000 dilution
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg
Lane 2:
293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 130 kDa
true
- WB
Supplier Data
Western blot - Anti-SAP97 antibody [EPR26467-123] (BSA and Azide free) (AB300482)
This data was developed using ab300481, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The blot of lane 6 was developed using a high sensitivity ECL substrate.The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8755482).
All lanes:
Western blot - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/1000 dilution
Lane 1:
U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 20 µg
Lane 2:
SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg
Lane 3:
Mouse brain tissue lysate 20 µg
Lane 4:
Mouse liver tissue lysate 20 µg
Lane 5:
Rat brain tissue lysate 20 µg
Lane 6:
Human cerebellum tissue lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 130 kDa
false
関連する標識済み抗体及び組成の異なる製品 (1)
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Anti-SAP97 antibody [EPR26467-123]
Reactivity data
製品の詳細
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
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補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of SAP97 is closely linked to synaptic plasticity and signal transduction. This protein forms part of larger protein complexes that include ion channels and receptors helping regulate the transmission of signals between neurons. In the brain SAP97 facilitates the clustering of neurotransmitter receptors at synapses including AMPA receptors which are vital for excitatory synaptic transmission. Its role extends to modulating the structural organization and functional regulation of these protein complexes at synaptic junctions.
Pathways
SAP97 is involved in key signaling processes particularly the glutamatergic synapse pathway where it influences the trafficking and surface expression of glutamate receptors. It interacts with other MAGUK family members like PSD-95 to regulate the assembly of the postsynaptic density. This protein also plays a role in the cardiac signaling cascade particularly in interactions involving potassium channels impacting the cardiac action potential.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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