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リコンビナントRabMAb

Anti-SAP97 抗体 [EPR26467-123] (ab300481)

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Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)
  • Anti-SAP97 antibody [EPR26467-123] (AB300481)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26467-123] to SAP97
  • Suitable for: IHC-Fr, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SAP97 antibody [EPR26467-123]
    SAP97 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26467-123] to SAP97

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, WB, IHC-P, IPmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 transfected with scrambled siRNA control and siRNAs specifically targeting DLG1, whole cell lysates; U-87 MG, SH-SY5Y, HeLa and 293T whole cell lysates; mouse brain and liver, rat brain and human cerebellum tissue lysates. IHC-P: Human colon, mouse and rat cerebrum, mouse liver FFPE tissue sections. IHC-Fr: Mouse and rat cerebrum fresh frozen tissues IP: SH-SY5Y whole cell lysate, mouse brain tissue lysate

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300481の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/100.
WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
特記事項
IHC-Fr
1/100.
WB
1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 100 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 機能

    Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.

  • 組織特異性

    Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.

  • 配列類似性

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 1 L27 domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.

  • ドメイン

    The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
    The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
    The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.

  • 翻訳後修飾

    Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A.

  • 細胞内局在

    Membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Discs large homolog 1 antibody
    • discs large, Drosophila, homolog of, 1 antibody
    • discs, large homolog 1 (Drosophila) antibody
    • Disks large homolog 1 antibody
    • dJ1061C18.1.1 antibody
    • DKFZp761P0818 antibody
    • DKFZp781B0426 antibody
    • DLG 1 antibody
    • DLG1 antibody
    • DLG1_HUMAN antibody
    • DLGH 1 antibody
    • DLGH1 antibody
    • hDlg antibody
    • Presynaptic protein SAP97 antibody
    • SAP 97 antibody
    • SAP-97 antibody
    • SAP97 antibody
    • Synapse associated protein 97 antibody
    • Synapse-associated protein 97 antibody
    see all

画像

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    All lanes : Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
    Lane 2 : HepG2 transfected with siRNAs specifically targeting DLG1, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 100 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Western blot - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    All lanes : Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Human cerebellum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 100 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:

    Lane 1: 103 seconds
    Lane 2: 3 minutes
    Lane 3-5: 26 seconds
    Lane 6: 48 seconds

    The blot of lane 6 was developed using a high sensitivity ECL substrate.
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8755482).

  • Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)
    Western blot - Anti-SAP97 antibody [EPR26467-123] (ab300481)
    All lanes : Anti-SAP97 antibody [EPR26467-123] (ab300481) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 100 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time:

    Lane 1: 81 seconds

    Lane 2: 36 seconds

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SAP97 with ab300481 at 1/2000 dilution (0.234 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on human colon (PMID: 7891172). The section was incubated with ab300481 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SAP97 with ab300481 at 1/4000 dilution (0.117 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on mouse cerebrum (PMID: 7891172, PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SAP97 with ab300481 at 1/4000 dilution (0.117 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on rat cerebrum (PMID: 7891172, PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAP97 with ab300481 at 1/4000 dilution (0.117 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on mouse liver (PMID: 11181181). The section was incubated with ab300481 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 µml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunohistochemistry (Frozen sections) - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling SAP97 with ab300481 at 1/100 (4.67 µml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

  • Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    SAP97 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg (Inset)

    Lane 2: ab300481 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300481 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

  • Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (AB300481)

    SAP97 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg (Inset)

    Lane 2: ab300481 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300481 in mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

  • Anti-SAP97 antibody [EPR26467-123] (AB300481)
    Anti-SAP97 antibody [EPR26467-123] (AB300481)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300481 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300481 は論文での使用が確認できていません。

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