Anti-SAP102 抗体 (ab152132)
Key features and details
- Rabbit polyclonal to SAP102
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SAP102 antibody
SAP102 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SAP102 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 525-803 of Human SAP102.
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, Mouse brain cell lysates; A431 cells; A549 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 90 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 90 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Required for learning most likely through its role in synaptic plasticity following NMDA receptor signaling. -
関連疾患
Defects in DLG3 are the cause of mental retardation X-linked type 90 (MRX90) [MIM:300189]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 509882 Cow
- Entrez Gene: 1741 Human
- Entrez Gene: 53310 Mouse
- Omim: 300189 Human
- SwissProt: Q92796 Human
- SwissProt: P70175 Mouse
- Unigene: 726128 Human
- Unigene: 4615 Mouse
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別名
- bA291O7.3 antibody
- bA291O7.3 discs large Drosophila homolog 3 neuroendocrine dlg antibody
- Discs large Drosophila homolog 3 antibody
see all
画像
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Western blot - Anti-SAP102 antibody (ab152132)Anti-SAP102 antibody (ab152132) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 90 kDa
7.5% SDS PAGE -
Western blot - Anti-SAP102 antibody (ab152132)Anti-SAP102 antibody (ab152132) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 90 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAP102 antibody (ab152132)Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling SAP102 with ab152132 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-SAP102 antibody (ab152132)Immunofluorescent analysis of methanol-fixed A431 cells labeling SAP102 with ab152132 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab152132 は 1 報の論文で使用されています。
- Tanaka M et al. Enhanced phosphorylation of PERK in primary cultured neurons as an autonomous neuronal response to prion infection. PLoS One 15:e0234147 (2020). PubMed: 32479530