AB76471

Anti-SAM68 抗体 [EPR3231]

Name: Anti-SAM68 抗体 [EPR3231] (ab76471) | Abcam
Brand: Abcam Limited
SKU: AB76471
Price: 26000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-SAM68 antibody [EPR3231]

RabMAb
Recombinant
20ul selling size
詳細を見る

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal SAM68 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

別名を表示する

SAM68, KHDRBS1, GAP-associated tyrosine phosphoprotein p62, Src-associated in mitosis 68 kDa protein, p21 Ras GTPase-activating protein-associated p62, p68, Sam68

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3231] (AB76471)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3231] (AB76471)

Immunofluorescent staining of HeLa cells using ab76471 at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3231] (AB76471)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3231] (AB76471)

ab76471 at 1/100 dilution staining SAM68 in human brain tissue using a HRP/AP polymerized secondary antibody.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SAM68 antibody [EPR3231] (AB76471)

All lanes:

Western blot - Anti-SAM68 antibody [EPR3231] (ab76471) at 1/100000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 68 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3231

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000 - 1/100000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein SAM68 also known as Src-associated in mitosis 68 kDa is a member of the STAR (Signal Transduction and Activation of RNA) family and has a mass of approximately 68 kilodaltons. SAM68 is expressed in a broad range of tissues but shows higher expression in the brain and testis. It functions mechanically as a nuclear RNA-binding protein involved in RNA metabolism alternative splicing and is linked to signal transduction pathways. The ability of SAM68 to bind RNA is important for its role in RNA processing.

Biological function summary

SAM68 impacts multiple cellular processes by regulating alternative splicing of pre-mRNA and is known to be part of a larger ribonucleoprotein complex. This functionality enables SAM68 to influence gene expression and impact cell proliferation and differentiation. By interacting with several signaling molecules it modulates processes that are vital for the proper functioning of various cell types including neurons and germ cells.

Pathways

SAM68 interacts with several signaling pathways notably the PI3K/AKT and MAPK/ERK pathways where it influences cellular survival and growth. Within these pathways SAM68 interacts with proteins such as the tyrosine kinase Src which facilitates signal transduction events leading to changes in cell behavior. The protein also associates with hnRNPA1 affecting mRNA processing and influencing downstream signaling cascades.

SAM68 has connections to cancer and neurodegenerative diseases. Abnormal expression or mutations in SAM68 are linked to oncogenesis particularly in prostate and breast cancers where it interacts with signaling proteins like ERK. In neurodegenerative disorders SAM68 is implicated in altering neuronal RNA processing potentially contributing to diseases such as Alzheimer's with connections to proteins like tau. Understanding SAM68's role in these diseases highlights its potential as a therapeutic target.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed : 22253824). RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner (PubMed : 26080397). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (PubMed : 17371836, PubMed : 20186123). Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (By similarity).. Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase.

See full target information KHDRBS1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:660 PubMed37349576

2023

The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Hui Yeo,Vignesh Sundararajan,Zhengwei Wu,Zi Jin Cheryl Phua,Yin Ying Ho,Kai Lay Esther Peh,Yi-Chia Chiu,Tuan Zea Tan,Dennis Kappei,Ying Swan Ho,David Shao Peng Tan,Wai Leong Tam,Ruby Yun-Ju Huang

Oncology reports 48: PubMed36082807

2022

High expression of Sam68 contributes to metastasis by regulating vimentin expression and a motile phenotype in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Komiyama,Takeshi Kuroshima,Takehito Sugasawa,Shin-Ichiro Fujita,Yuta Ikami,Hideaki Hirai,Fumihiko Tsushima,Yasuyuki Michi,Kou Kayamori,Fumihiro Higashino,Hiroyuki Harada

PloS one 7:e29921 PubMed22253824

2012

Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA.

Applications

Species

Human

Kalpana Rajanala,Vinay Kumar Nandicoori

PloS one 6:e20147 PubMed21647438

2011

Nitric oxide synthase inhibition enhances the antitumor effect of radiation in the treatment of squamous carcinoma xenografts.

Applications

IHC

Species

Unspecified reactive species

Robert J G Cardnell,Ross B Mikkelsen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SAM68 抗体 [EPR3231]

Anti-SAM68 antibody [EPR3231]

Immunocytochemistry/ Immunofluorescence - Anti-SAM68 antibody [EPR3231] (AB76471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAM68 antibody [EPR3231] (AB76471)

Western blot - Anti-SAM68 antibody [EPR3231] (AB76471)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise