Anti-Salmonella typhimurium LPS 抗体 [1E6] (ab8274)

Unfortunately, we do not carry the biotinylated forms of these 2 antibodies, but we do carry biotinylation kits (e.g. https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html - other sizes exist as well) if you like to biotinylate the antibodies on your own.

We have 5 antibodies to Salmonella typhimurium:

ab69238 - was raised against the whole bacteria thus we do not know where the antibody would bind.

ab8274 and ab65922: both were raised against LPS of the bacterium which I suppose is located in the bacterial wall and not the flagellum - this should work (https://www.abcam.com/salmonella-typhimurium-0-4-antibody-1e6-ab8274.html)
(https://www.abcam.com/salmonella-typhimurium-lps-antibody-1e6-ab65922.html)

ab63901: was raised against the bacteria, but known to be reactive with the LPS - thus, it should work.

ab128709: raised against purified LPS from Salmonella typhimurium and reacts with an unidentified LPS determinant of Salmonella typhimurium - thus it should work for your purposes (https://www.abcam.com/Salmonella-typhimurium-LPS-antibody-1E6-ab128709.html).

Thank you for contacting Abcam.

I have no added both Salmonella Typhimurium and Salmonella Enteritidis to our list of species and so you should now be able to submit anAbreview for this antibody.

If there is anything else I can help you with, please let me know.

DISCOUNT CODE: ****** Expiration date:****** I am very pleased to hear you would like to accept our offer and test ab8274 in western blot. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich immunoassay. (The best pair for sandwich ELISA was selected among the antibodies by testing all possible pairs). Unfortunately we have available only one antibody clone for the detection of Salmonella typhimurium. However, we are also offering antibodies for the detection of A, B and D-group specific salmonella O-antigens. We have information from a customer that they have used successfully the pair 10D9 (ab8272) – 5D12A (ab8273) (capture – conjugate) in immunochromatography for the detection of salmonella from food. The detection antibodies are available in conjugated form (e.g. hrp-conjugated). We are very sorry we do not have references for those antibodies. Here is the recommended protocol for Sandwich ELISA: Coating: Capture MAbs, 2.5 µg/ml in 0.1 M carbonate buffer, pH 9.3, overnight at + 4 C. Antigen: L. monocytogenes OM fraction or cell suspension, 1 h, 37 C. Conjugate: Conjugated MAbs in PBS-AT, 1 h, 37 C. Substrate: TMB, 15 min Using pair LZH1 – LZF7 the detection limits 0.3 ng/ml for L. monocytogenes OM fraction and 100 cells per well for L. monocytogenes cell suspension have been achieved. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (capture – conjugate) for sandwich immunoassay. (The best pair for sandwich ELISA was selected among the antibodies by testing all possible pairs). Unfortunately we have available only one antibody clone for the detection of Salmonella typhimurium. However, we are also offering antibodies for the detection of A, B and D-group specific salmonella O-antigens. We have information from a customer that they have used successfully the pair 10D9 (ab8272) – 5D12A (ab8273) (capture – conjugate) in immunochromatography for the detection of salmonella from food. The detection antibodies are available in conjugated form (e.g. hrp-conjugated). We are very sorry we do not have references for those antibodies. Sandwich ELISA Protocol: Coating: Capture MAbs, 2.5 µg/ml in 0.1 M carbonate buffer, pH 9.3, overnight at + 4 C. Antigen: L. monocytogenes OM fraction or cell suspension, 1 h, 37 C. Conjugate: Conjugated MAbs in PBS-AT, 1 h, 37 C. Substrate: TMB, 15 min Using pair LZH1 – LZF7 the detection limits 0.3 ng/ml for L. monocytogenes OM fraction and 100 cells per well for L. monocytogenes cell suspension have been achieved. Please do not hesitate to contact us again if you need further assistance or the HRP conjugated detection antibodies and we will add them to the catalogue and provide you with their catalogue number.

Both monoclonal antibodies recognize heat-killed Salmonella typhimurium. We have used the monoclonal antibodies (Mab) in ELISA according to the following scheme: antigen coating - Mab - Anti-mouse-IgG (conjugated), not in sandwich ELISA. The antibodies may be used for salmonella typhimurium detection in dot-blot, ELISA and immunofluorescence. Other applications have not been tested. Both clones do not recognise: S.paratyphi A, S.choleraesuis, S.newport, S. enteiditis, S. anatum, S. elandia, E.coli 055:B5, E.coli K12, Klebsiela pneum, Proteus vulgaris. Ab8274 has been shown to have a very broad cross-reactivity range recognising E.coli 1243 and Listeria monocytogenes species, being a potential positive control antibody for a variety of assays.