Anti-SAE1 抗体 [EPR15398] (ab185552)
![Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-219931-anti-sae1-antibody-epr15398-western-blot.jpg "Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15398] to SAE1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-SAE1 antibody [EPR15398]
SAE1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15398] to SAE1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Hela, Jurkat, 293 & MCF-7 cell lysates, Hela & MCF-7 cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15398 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/230.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
|
| ICC/IF |
1/500.
|
|
| IP |
1/50 - 1/70.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/230. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 38 kDa. |
|
ICC/IF
1/500. |
|
IP
1/50 - 1/70. |
ターゲット情報
-
機能
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
組織特異性
Expression level increases during S phase and drops in G2 phase (at protein level). -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the ubiquitin-activating E1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 10055 Human
- Entrez Gene: 308384 Rat
- Omim: 613294 Human
- SwissProt: Q9UBE0 Human
- SwissProt: Q6AXQ0 Rat
- Unigene: 515500 Human
- Unigene: 9014 Rat
-
別名
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
画像
-
Western blot - Anti-SAE1 antibody [EPR15398] (ab185552)All lanes : Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDaBlocking buffer: 5% NFDM/TBST
-
![Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-219935-anti-sae1-antibody-epr15398-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SAE1 antibody [EPR15398] (ab185552)Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking buffer: 5% NFDM/TBST.
-
![Flow Cytometry (Intracellular) - Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-219934-anti-sae1-antibody-epr15398-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SAE1 antibody [EPR15398] (ab185552)Intracellular Flow Cytometry analysis of HeLa cells using ab185552 at 1/230 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-219933-anti-sae1-antibody-epr15398-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-219932-anti-sae1-antibody-epr15398-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SAE1 antibody [EPR15398] (ab185552)Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
-
![Anti-SAE1 antibody [EPR15398] (ab185552)](https://www.abcam.co.jp/ps/products/185/ab185552/Images/ab185552-6-benefits-of-recombinant-antibodies.png)
Anti-SAE1 antibody [EPR15398] (ab185552)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (8)
ab185552 は 8 報の論文で使用されています。
- Tan D et al. SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways. Nat Commun 14:3887 (2023). PubMed: 37393345
- Sheng H et al. Construction and validation of a two-gene signature based on SUMOylation regulatory genes in non-small cell lung cancer patients. BMC Cancer 22:572 (2022). PubMed: 35606717
- Ong JR et al. SUMO-Activating Enzyme Subunit 1 (SAE1) Is a Promising Diagnostic Cancer Metabolism Biomarker of Hepatocellular Carcinoma. Cells 10:N/A (2021). PubMed: 33477333
- Du MG et al. The Absence of PTEN in Breast Cancer Is a Driver of MLN4924 Resistance. Front Cell Dev Biol 9:667435 (2021). PubMed: 33996822
- Chen Z et al. Linking nuclear matrix-localized PIAS1 to chromatin SUMOylation via direct binding of histones H3 and H2A.Z. J Biol Chem 297:101200 (2021). PubMed: 34537242
- Wu R et al. SUMOylation of the transcription factor ZFHX3 at Lys-2806 requires SAE1, UBC9, and PIAS2 and enhances its stability and function in cell proliferation. J Biol Chem 295:6741-6753 (2020). PubMed: 32249212
- Yang Y et al. SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways. Cell Commun Signal 17:82 (2019). PubMed: 31345225
- Wang F et al. Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations. Osteoarthritis Cartilage 24:1113-24 (2016). PubMed: 26826302