Anti-Sacsin 抗体 [EPR11864] (ab181190)
![Western blot - Anti-Sacsin antibody [EPR11864] (ab181190)](https://www.abcam.co.jp/ps/products/181/ab181190/Images/ab181190-214669-anti-sacsin-antibody-epr11864-western-blot.png "Western blot - Anti-Sacsin antibody [EPR11864] (ab181190)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11864] to Sacsin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Sacsin antibody [EPR11864]
Sacsin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11864] to Sacsin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal brain lysate. Human glioma lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11864 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 521 kDa.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 521 kDa. |
ターゲット情報
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機能
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins. -
組織特異性
Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas. -
関連疾患
Spastic ataxia Charlevoix-Saguenay type -
配列類似性
Contains 1 HEPN domain.
Contains 1 J domain.
Contains 1 ubiquitin-like domain. -
ドメイン
The ubiquitin-like domain mediates interaction with the proteasome.
The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity. -
細胞内局在
Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60. - Information by UniProt
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参照データベース
- Entrez Gene: 26278 Human
- Omim: 604490 Human
- SwissProt: Q9NZJ4 Human
- Unigene: 159492 Human
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別名
- DnaJ homolog subfamily C member 29 antibody
- DNAJC29 antibody
- SACS antibody
see all
画像
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Western blot - Anti-Sacsin antibody [EPR11864] (ab181190)Anti-Sacsin antibody [EPR11864] (ab181190) at 1/5000 dilution + Human glioma tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform) -
![Western blot - Anti-Sacsin antibody [EPR11864] (ab181190)](https://www.abcam.co.jp/ps/products/181/ab181190/Images/ab181190-214668-anti-sacsin-antibody-epr11864-western-blot.png)
Western blot - Anti-Sacsin antibody [EPR11864] (ab181190)Anti-Sacsin antibody [EPR11864] (ab181190) at 1/2000 dilution + Human fetal brain at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform) -
![Anti-Sacsin antibody [EPR11864] (ab181190)](https://www.abcam.co.jp/ps/products/181/ab181190/Images/ab181190-3-benefits-of-recombinant-antibodies.png)
Anti-Sacsin antibody [EPR11864] (ab181190)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab181190 は 3 報の論文で使用されています。
- Romano LEL et al. Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization. Cell Rep 41:111580 (2022). PubMed: 36323248
- Longo F et al. Assessment of Sacsin Turnover in Patients With ARSACS: Implications for Molecular Diagnosis and Pathogenesis. Neurology 97:e2315-e2327 (2021). PubMed: 34649874
- Larivière R et al. Sacs R272C missense homozygous mice develop an ataxia phenotype. Mol Brain 12:19 (2019). PubMed: 30866998