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リコンビナントRabMAb

Anti-Sacsin 抗体 [EPR11864-61] (ab181207)

Reviews (2) Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] (ab181207)
  • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
  • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
  • Anti-Sacsin antibody [EPR11864-61] (ab181207)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11864-61] to Sacsin
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Sacsin antibody [EPR11864-61]
    Sacsin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11864-61] to Sacsin

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human fetal brain, Human glioma and HeLa cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181207の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/470.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/20000. Detects a band of approximately 437 kDa (predicted molecular weight: 521 kDa).
特記事項
Flow Cyt (Intra)
1/470.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/20000. Detects a band of approximately 437 kDa (predicted molecular weight: 521 kDa).

ターゲット情報

  • 機能

    Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins.

  • 組織特異性

    Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas.

  • 関連疾患

    Spastic ataxia Charlevoix-Saguenay type

  • 配列類似性

    Contains 1 HEPN domain.
    Contains 1 J domain.
    Contains 1 ubiquitin-like domain.

  • ドメイン

    The ubiquitin-like domain mediates interaction with the proteasome.
    The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity.

  • 細胞内局在

    Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DnaJ homolog subfamily C member 29 antibody
      • DNAJC29 antibody
      • SACS antibody
      • SACS_HUMAN antibody
      • Sacsin antibody
      • Spastic ataxia of Charlevoix-Saguenay antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] (ab181207)
      Flow Cytometry (Intracellular) - Anti-Sacsin antibody [EPR11864-61] (ab181207)

      Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Sacsinwith ab181207at 1/470 dilution (red), or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) was used as a secondary antibody at 1/150 dilution. 

       

       

    • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
      Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
      All lanes : Anti-Sacsin antibody [EPR11864-61] (ab181207) at 1/5000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human glioma cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 521 kDa



      Blocking/diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
      Western blot - Anti-Sacsin antibody [EPR11864-61] (ab181207)
      Anti-Sacsin antibody [EPR11864-61] (ab181207) at 1/20000 dilution + HeLa cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 521 kDa

    • Anti-Sacsin antibody [EPR11864-61] (ab181207)
      Anti-Sacsin antibody [EPR11864-61] (ab181207)

    参考文献 (1)

    ab181207 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181207 は 1 報の論文で使用されています。

    • Larivière R  et al. Sacs R272C missense homozygous mice develop an ataxia phenotype. Mol Brain 12:19 (2019). PubMed: 30866998

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Sacsin antibody [EPR11864-61]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (cerebellum)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 01 2017

    Western blot abreview for Anti-Sacsin antibody [EPR11864-61]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (cerebellum)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 30 2017

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