AB4464

Anti-SA2 抗体

Name: Anti-SA2 抗体 (ab4464) | Abcam
Brand: Abcam Limited
SKU: AB4464
Price: 76000 JPY
Availability: InStock

Anti-SA2 antibody

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(5 Publications)

Goat Polyclonal SA2 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human STAG2.

別名を表示する

SA2, STAG2, Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2

2 Images

Unknown

Immunocytochemistry - Anti-SA2 antibody (AB4464)

Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4464 at 1µg/ml.

Supplier Data

Western blot - Anti-SA2 antibody (AB4464)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-SA2 antibody (ab4464) at 1/10000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

Jurkat Whole cell lysate at 50 µg

Lane 4:

mouse TCMK-1 cells Whole cell lysate at 50 µg

Predicted band size: 141 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC, WB

applications

免疫原

Synthetic Peptide within Human STAG2. The exact immunogen used to generate this antibody is proprietary information.

Q8N3U4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/500 - 1/2000", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.

Biological function summary

SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.

Pathways

SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.

SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

See full target information STAG2

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1859 PubMed35388001

2022

STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaowei Chu,Lei Gu,Yeguang Hu,Xiaoyang Zhang,Man Li,Jiajia Chen,Da Teng,Man Huang,Che-Hung Shen,Li Cai,Toshimi Yoshida,Yifeng Qi,Zhixin Niu,Austin Feng,Songmei Geng,Dennie T Frederick,Emma Specht,Adriano Piris,Ryan J Sullivan,Keith T Flaherty,Genevieve M Boland,Katia Georgopoulos,David Liu,Yang Shi,Bin Zheng

Molecular cell 82:833-851.e11 PubMed35180428

2022

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Huacheng Luo,Ganqian Zhu,Melanie A Eshelman,Tsz Kan Fung,Qian Lai,Fei Wang,Bernd B Zeisig,Julia Lesperance,Xiaoyan Ma,Shi Chen,Nicholas Cesari,Christopher Cogle,Baoan Chen,Bing Xu,Feng-Chun Yang,Chi Wai Eric So,Yi Qiu,Mingjiang Xu,Suming Huang

Nature communications 10:5718 PubMed31844045

2019

ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Su Hyung Park,Nalae Kang,Eunho Song,Minwoo Wie,Eun A Lee,Sunyoung Hwang,Deokjae Lee,Jae Sun Ra,In Bae Park,Jieun Park,Sukhyun Kang,Jun Hong Park,Sungchul Hohng,Kyoo-Young Lee,Kyungjae Myung

Genome research 22:2163-75 PubMed22780989

2012

Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules.

Applications

ChIP-seq

Species

Mouse

Andre J Faure,Dominic Schmidt,Stephen Watt,Petra C Schwalie,Michael D Wilson,Huiling Xu,Robert G Ramsay,Duncan T Odom,Paul Flicek

Cell cycle (Georgetown, Tex.) 6:1646-54 PubMed17611414

2007

Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Inoue,Tongyuan Li,Sarah K Roby,Marcus B Valentine,Madoka Inoue,Kelli Boyd,Vincent J Kidd,Jill M Lahti

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-SA2 抗体

Anti-SA2 antibody

Immunocytochemistry - Anti-SA2 antibody (AB4464)

Western blot - Anti-SA2 antibody (AB4464)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

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Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise