Anti-SA1 抗体 - C-terminal

• IP

Supplier Data

Immunoprecipitation - Anti-SA1 antibody - C-terminal (AB227736)

SA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg of ab227736. Western blot was performed from the immunoprecipitate using ab227736.

Lane 1 : HeLa whole cell extract (Input).

Lane 2 : Control IgG instaed of ab227736 in HeLa whole cell extract.

Lane 3 : ab227736 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-SA1 antibody - C-terminal (ab227736)

Predicted band size: 144 kDa

false

• WB

Supplier Data

Western blot - Anti-SA1 antibody - C-terminal (AB227736)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SA1 antibody - C-terminal (ab227736) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 144 kDa

false

• WB

Supplier Data

Western blot - Anti-SA1 antibody - C-terminal (AB227736)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SA1 antibody - C-terminal (ab227736) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SA1 shRNA, whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 144 kDa

false