Anti-SA1 抗体 (ab4455)
Key features and details
- Goat polyclonal to SA1
- Suitable for: IP, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SA1 antibody
SA1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SA1 -
由来種
Goat -
アプリケーション
適用あり: IP, WB, ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rhesus monkey
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免疫原
Synthetic peptide within Human SA1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005853.2 (GeneID 10274).
Database link: Q8WVM7 -
ポジティブ･コントロール
- WB: HeLa nuclear extract. HeLa, MCF-7, HEK-293T, TCMK-1 and NIH/3T3 whole cell lysate. ICC: HeLa cells. IP: SA1 IP in HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4455の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
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| ICC |
1/500 - 1/2000.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa). |
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ICC
1/500 - 1/2000. |
ターゲット情報
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機能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
配列類似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻訳後修飾
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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参照データベース
- Entrez Gene: 10274 Human
- Entrez Gene: 20842 Mouse
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9D3E6 Mouse
- Unigene: 412586 Human
- Unigene: 42135 Mouse
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別名
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
画像
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Western blot - Anti-SA1 antibody (ab4455)
Whole cell lysate (15 µg) from HeLa, MCF-7, HEK-293T, TCMK-1, and NIH/3T3 cells prepared using NETN lysis buffer.
ab4455 used for WB at 0.1 µg/ml.
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Immunoprecipitation - Anti-SA1 antibody (ab4455)Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: ab4455 used for IP at 6 µg per reaction.
Lane 2: Control IgG.
For blotting immunoprecipitated SA1, ab4455 was used at 0.1 µg/ml.
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Western blot - Anti-SA1 antibody (ab4455)Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure. -
Immunocytochemistry - Anti-SA1 antibody (ab4455)Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab4455 は 5 報の論文で使用されています。
- Porter H et al. Cohesin-independent STAG proteins interact with RNA and R-loops and promote complex loading. Elife 12:N/A (2023). PubMed: 37010886
- Tian H et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). WB ; Mouse . PubMed: 24992337
- Xiao T et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
- Leman AR et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP ; Human . PubMed: 20124417