Anti-S1P1/EDG1 (phospho T236) 抗体 (ab111571)
Key features and details
- Rabbit polyclonal to S1P1/EDG1 (phospho T236)
- Suitable for: ICC/IF
- Reacts with: African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-S1P1/EDG1 (phospho T236) antibody
S1P1/EDG1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S1P1/EDG1 (phospho T236) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: African green monkey
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human S1P1/EDG1 (phospho T236).
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ポジティブ･コントロール
- COS-7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab111571 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111571の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
組織特異性
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- SwissProt: O08530 Mouse
- SwissProt: P48303 Rat
- Unigene: 982 Mouse
- Unigene: 109455 Rat
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別名
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 (phospho T236) antibody (ab111571)
ab111571, at 1/100 dilution, staining S1P1/EDG1 in COS-7 cells by Immunofluorescence, in the presence (right panel) or absence (left panel) of immunising peptide.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab111571 は 1 報の論文で使用されています。
- Yang CH et al. Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain. Cell Rep 30:959-968.e3 (2020). PubMed: 31995766