Anti-S1P1/EDG1 抗体 (ab77076)
Key features and details
- Rabbit polyclonal to S1P1/EDG1
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-S1P1/EDG1 antibody
S1P1/EDG1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S1P1/EDG1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- mouse thymus tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77076の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa.
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| IHC-P | (2) |
Use a concentration of 2.5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 42 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
組織特異性
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 13609 Mouse
- SwissProt: O08530 Mouse
- Unigene: 982 Mouse
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別名
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
画像
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Western blot - Anti-S1P1/EDG1 antibody (ab77076)Lane 1 : Anti-S1P1/EDG1 antibody (ab77076) at 1 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab77076) at 2 µg/ml
All lanes : mouse thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunofluorescence of S1P1/EDG1 in mouse thymus tissue with ab77076 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunohistochemistry of S1P1/EDG1 in mouse thymus tissue with ab77076 at 5 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody (ab77076)Immunohistochemistry of S1P1/EDG1 in mouse thymus tissue with ab77076 at 2.5 μg/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab77076 は 6 報の論文で使用されています。
- Yang CH et al. Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain. Cell Rep 30:959-968.e3 (2020). PubMed: 31995766
- Li Y et al. The imbalance in the aortic ceramide/sphingosine-1-phosphate rheostat in ovariectomized rats and the preventive effect of estrogen. Lipids Health Dis 19:95 (2020). PubMed: 32430006
- Chen A et al. Inhibition of miR-155-5p attenuates the valvular damage induced by rheumatic heart disease. Int J Mol Med 45:429-440 (2020). PubMed: 31894293
- Ji Y et al. Potential biomarkers Ang II/AT1R and S1P/S1PR1 predict the prognosis of hepatocellular carcinoma. Oncol Lett 20:208 (2020). PubMed: 32963614
- Guan Z et al. Sphingosine-1-Phosphate Evokes Unique Segment-Specific Vasoconstriction of the Renal Microvasculature. J Am Soc Nephrol N/A:N/A (2014). PubMed: 24578134
- Zhu Q et al. Low-dose cytokine-induced neutral ceramidase secretion from INS-1 cells via exosomes and its anti-apoptotic effect. FEBS J 281:2861-70 (2014). PubMed: 24798654