Anti-S1P 抗体 (ab59870)
Key features and details
- Rabbit polyclonal to S1P
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-S1P antibody
S1P 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to S1P -
由来種
Rabbit -
特異性
We have a range of domain specific antibodies for this target. For a full list please see all MBTPS1 antibodies. ab59870 recognizes all four forms of MBTPS1 (1052, 746, 607 and 552 amino acids in length). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide based on the catalytic domain of human MBTPS1. Immunizing peptide available on request - please contact a member of staff to ask for availability.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 118 kDa. 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. |
ターゲット情報
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関連性
S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain. -
細胞内局在
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 8720 Human
- Omim: 603355 Human
- SwissProt: Q14703 Human
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別名
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
see all
画像
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Western blot - Anti-S1P antibody (ab59870)Anti-S1P antibody (ab59870) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Exposure time: 16 minutes
The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab59870 は 2 報の論文で使用されています。
- Chen Y et al. SPK1/S1P axis confers gastrointestinal stromal tumors (GISTs) resistance of imatinib. Gastric Cancer 26:26-43 (2023). PubMed: 35999321
- Tang W et al. Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease. Hum Mol Genet 32:1466-1482 (2023). PubMed: 36519761