Anti-S100A9 抗体 [47-8D3] (ab24111)
Key features and details
- Mouse monoclonal [47-8D3] to S100A9
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-S100A9 antibody [47-8D3]
S100A9 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [47-8D3] to S100A9 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Tissue/ cell preparation of peripheral blood monocyte components (Human).
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ポジティブ・コントロール
- Lysate of human monocytes or granulocytes.
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特記事項
Can be used for phenotypical characterization of macrophages and myelomonocytic cells in situ.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
Can be used for phenotypical characterization of macrophages and myelomonocytic cells in situ. -
ポリ/モノ
モノクローナル -
クローン名
47-8D3 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
組織特異性
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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参照データベース
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
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別名
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab24111 は 7 報の論文で使用されています。
- Sill M et al. Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays. Microarrays (Basel) 5:N/A (2016). IHC . PubMed: 27600085
- Sun W et al. Quantitative Proteomics Analysis of Tissue Interstitial Fluid for Identification of Novel Serum Candidate Diagnostic Marker for Hepatocellular Carcinoma. Sci Rep 6:26499 (2016). WB . PubMed: 27216119
- Gschwandtner M et al. Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes. J Invest Dermatol N/A:N/A (2014). PubMed: 24694903
- Fornander L et al. Airway symptoms and biological markers in nasal lavage fluid in subjects exposed to metalworking fluids. PLoS One 8:e83089 (2013). WB . PubMed: 24391738
- Gromov P et al. Up-regulated proteins in the fluid bathing the tumour cell microenvironment as potential serological markers for early detection of cancer of the breast. Mol Oncol 4:65-89 (2010). IHC-P ; Human . PubMed: 20005186
- Abtin A et al. The antimicrobial heterodimer S100A8/S100A9 (calprotectin) is upregulated by bacterial flagellin in human epidermal keratinocytes. J Invest Dermatol 130:2423-30 (2010). PubMed: 20555353
- Dowling P et al. Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis. J Proteomics 71:168-75 (2008). PubMed: 18617144