Anti-S100A9 + Calprotectin (S100A8/A9 complex) 抗体 [MAC387] (ab22506)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)](https://www.abcam.co.jp/ps/products/22/ab22506/Images/ab22506-260173-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)")
Key features and details
- Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387]
S100A9 + Calprotectin (S100A8/A9 complex) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex) -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Monkey, Baboon
非交差種:
Rat -
免疫原
Tissue, cells or virus corresponding to Human S100A9 + Calprotectin (S100A8/A9 complex). Affinity purified solubilized human monocyte preparation.
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ポジティブ･コントロール
- IHC-P: Human Hodgkin's lymphoma tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
MAC387 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (18) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)This image is courtesy of an Abreview submitted by Elizabeth Chlipala
ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)](https://www.abcam.co.jp/ps/products/22/ab22506/Images/ab22506-31856-anti-s100a9-calprotectin-s100a8-a9-complex-antibody-mac387-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (ab22506)IHC image of Macrophage staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22506, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (87)
ab22506 は 87 報の論文で使用されています。
- Hu X et al. Fibroblast Growth Factor 2 Is Produced By Renal Tubular Cells to Act as a Paracrine Factor in Maladaptive Kidney Repair After Cisplatin Nephrotoxicity. Lab Invest 103:100009 (2023). PubMed: 36925200
- Pop OT et al. AXL Expression on Homeostatic Resident Liver Macrophages Is Reduced in Cirrhosis Following GAS6 Production by Hepatic Stellate Cells. Cell Mol Gastroenterol Hepatol 16:17-37 (2023). PubMed: 37004869
- Kao KD et al. The Cxcr2+ subset of the S100a8+ gastric granylocytic myeloid-derived suppressor cell population (G-MDSC) regulates gastric pathology. Front Immunol 14:1147695 (2023). PubMed: 37744359
- Liu X et al. Urinary single-cell sequence analysis of the urinary macrophage in different outcomes of membranous nephropathy. Clin Kidney J 16:2405-2416 (2023). PubMed: 38046013
- Sinha S et al. Dexamethasone modulates immature neutrophils and interferon programming in severe COVID-19. Nat Med 28:201-211 (2022). PubMed: 34782790