Name: Anti-S100A10 antibody [EPR3317]
Brand: RabMAb
SKU: ab76472
Rating: 5 (3 reviews)

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Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3317] to S100A10
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品名

Anti-S100A10 antibody [EPR3317]
S100A10 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR3317] to S100A10
由来種

Rabbit
特異性

The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF
種交差性

交差種: Mouse, Human
免疫原

Synthetic peptide within Human S100A10 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P60903
ポジティブ･コントロール
- WB: HaCaT, A431, SW480, RAW264.7, NIH/3T3, and HT-29 cell lysates; IHC-P: Human squamous cell cervical carcinoma, colon tissue and human kidney tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR3317
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab76472の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/5000 - 1/50000. Predicted molecular weight: 11 kDa.
IHC-P	(2)	1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

特記事項
WB 1/5000 - 1/50000. Predicted molecular weight: 11 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

追加情報

Is unsuitable for Flow Cyt or ICC/IF.

機能

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
配列類似性

Belongs to the S-100 family.
Target information above from: UniProt accession P60903 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6281 Human
- Entrez Gene: 20194 Mouse
- Omim: 114085 Human
- SwissProt: P60903 Human
- SwissProt: P08207 Mouse
- Unigene: 143873 Human
- Unigene: 1 Mouse
別名
- 42C antibody
- AA409961 antibody
- AL024248 antibody
- Annexin II ligand antibody
- Annexin II ligand, calpactin I, light polypeptide antibody
- Annexin II tetramer (AIIt) p11 subunit antibody
- Annexin II, light chain antibody
- ANX2L antibody
- ANX2LG antibody
- Ca[1] antibody
- CAL12 antibody
- CAL1L antibody
- Calpactin I light chain antibody
- Calpactin I, p11 subunit antibody
- Calpactin-1 light chain antibody
- Cellular ligand of annexin II antibody
- CLP11 antibody
- GP11 antibody
- MGC111133 antibody
- Nerve growth factor-induced protein 42C antibody
- OTTHUMP00000015269 antibody
- OTTHUMP00000015270 antibody
- p10 antibody
- p10 protein antibody
- p11 antibody
- Protein S100 A10 antibody
- Protein S100-A10 antibody
- S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody
- S100 calcium binding protein A10 (calpactin) antibody
- S100 calcium binding protein A10 antibody
- S100 calcium-binding protein A10 antibody
- S100a10 antibody
- S10AA_HUMAN antibody
see all

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)

All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution (unpurified)

Lane 1 : HaCaT (Human skin keratinocyte) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding)

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)

Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)

Lane 1 : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution (purified)
Lane 2 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/5000 dilution

All lanes : Recombinant Human S100A10 protein (His tag) (ab221399)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa (possible dimer)

Exposure time: 3 seconds

Blocking buffer: 5% NFDM/TBST
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)

All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution (purified)

Lane 1 : HACAT cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HT-29 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)

All lanes : Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution (purified)

Lane 1 : RAW264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)

Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)

Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-S100A10 antibody [EPR3317] (ab76472)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab76472 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76472 は 11 報の論文で使用されています。

Yu J et al. Astragaloside IV inhibits experimental autoimmune encephalomyelitis by modulating the polarization of both microglia/macrophages and astrocytes. Folia Neuropathol 61:273-290 (2023). PubMed: 37818688
Oizumi H et al. Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout. iScience 26:107448 (2023). PubMed: 37720086
Wan T et al. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice. Nat Commun 13:1134 (2022). PubMed: 35241660
Wang S et al. CSE/H2S ameliorates colitis in mice via protection of enteric glial cells and inhibition of the RhoA/ROCK pathway. Front Immunol 13:966881 (2022). PubMed: 36189321
Gao Y et al. Proteomic analysis of human hippocampal subfields provides new insights into the pathogenesis of Alzheimer's disease and the role of glial cells. Brain Pathol 32:e13047 (2022). PubMed: 35016256

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 11 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 11 2022

Application

Western blot

Sample

Monkey Cell lysate - whole cell (COS7 cell)

Gel Running Conditions

Non-reduced Denaturing (15% gel)

Loading amount

10 µg

Specification

COS7 cell

Blocking step

blocking one(nacalai) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 18 2016

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (11)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A10 antibody [EPR3317]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A10 antibody [EPR3317]

Western blot abreview for Anti-S100A10 antibody [EPR3317]