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リコンビナントRabMAb

Anti-S100 beta 抗体 [SP127] - BSA and Azide free (ab242388)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Flow Cytometry (Intracellular) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
  • Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP127] to S100 beta - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-S100 beta antibody [SP127] - BSA and Azide free
    S100 beta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP127] to S100 beta - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Cow

  • 免疫原

    Recombinant full length protein corresponding to Human S100 beta.
    Database link: P04271

  • ポジティブ･コントロール

    • IHC-P: Human colon, melanoma, kidney, colon, spinal cord, cerebellum, adrenal gland bladder, breast, oesophagus, Mouse cerebrum, and Rat cerebrum tissue. Flow Cyt (Intra): A375 cells.

  • 特記事項

    ab242388 is the carrier-free version of ab115803.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab242388の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Incubate with primary antibody for 10 minutes at room temperature.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Incubate with primary antibody for 10 minutes at room temperature.

ターゲット情報

  • 機能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling.

  • 組織特異性

    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

  • 配列類似性

    Belongs to the S-101 family.
    Contains 2 EF-hand domains.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • NEF antibody
    • Protein S100 B antibody
    • Protein S100-B antibody
    • S 100 calcium binding protein beta chain antibody
    • S 100 protein beta chain antibody
    • S-100 protein beta chain antibody
    • S-100 protein subunit beta antibody
    • S100 antibody
    • S100 calcium binding protein beta (neural) antibody
    • S100 calcium-binding protein B antibody
    • S100 protein beta chain antibody
    • S100B antibody
    • S100B_HUMAN antibody
    • S100beta antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Flow Cytometry (Intracellular) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Flow Cytometry (Intracellular) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling S100 beta with purified ab115803 at 1:200 dilution (0.79 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab130748).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human esophagus tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human breast tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human cerebellum tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human spinal cord tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

    Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

  • Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)
    Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab242388 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab242388 は論文での使用が確認できていません。

レビューと Q&A

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com