AB227914

Anti-S100 beta 抗体 - Astrocyte Marker

Name: Anti-S100 beta 抗体 - Astrocyte Marker (ab227914) | Abcam
Brand: Abcam Limited
SKU: AB227914
Price: 56000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-S100 beta antibody - Astrocyte Marker

Lab Essentials
詳細を見る

(1 Review)

(4 Publications)

Rabbit Polyclonal S100 beta antibody. Astrocyte marker. Suitable for WB, IHC-P, IHC-Fr, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human S100B.

別名を表示する

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunofluorescence analysis of rat E18 primary cortical neuron and glia cells fixed in 4% paraformaldehyde at RT for 15 min labelling S100 beta protein at glia cells using ab227914 at a 1/500 dilution (Green). Tau, stained by anti-Tau antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining.

Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Frozen sections of adult mouse retina tissue stained for S100 beta using ab227914 at 1/250 dilution in immunohistochemical analysis.

Counter stain : beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/250 dilution (red).
Nuclear counter stain : Fluoroshield with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for S100 beta protein at cytoplasm with ab227914 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Paraffin-embedded mouse hind brain tissue stained for S100 beta using ab227914 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for S100 beta protein at cytoplasm with ab227914 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/500 dilution

All lanes:

A375 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 10 kDa

false

Supplier Data

Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with V5-human S100 beta, whole cell extract at 30 µg

Predicted band size: 10 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-Fr, IHC-P, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human S100B.

P04271

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.

Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed : 20950652, PubMed : 6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed : 20950652, PubMed : 6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed : 20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed : 22399290).

See full target information S100B

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 38:2002-2010 PubMed37219039

2023

TRIM29 facilitates proliferation and malignancy of cholangiocarcinoma cells by activating MAPK and β-catenin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Wenqing Bao,Haiyan Diao,Shaofei Yang,Lin Qiu,Chunjing Xu,Bin Zhao

Experimental neurology 352:114053 PubMed35341747

2022

Mycobacterium leprae induces Schwann cell proliferation and migration in a denervated milieu following intracutaneous excision axotomy in nine-banded armadillos.

Applications

Unspecified application

Species

Unspecified reactive species

Gigi J Ebenezer,Maria T Pena,Amrita S Daniel,Richard W Truman,Linda Adams,Malcolm S Duthie,Kelly Wagner,Serena Zampino,Eleanor Tolf,Daniel Tsottles,Michael Polydefkis

Acta neuropathologica communications 8:159 PubMed32912327

2020

Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Masataka Ifuku,Lukas Hinkelmann,Leonard D Kuhrt,Ibrahim E Efe,Victor Kumbol,Alice Buonfiglioli,Christina Krüger,Philipp Jordan,Marcus Fulde,Mami Noda,Helmut Kettenmann,Seija Lehnardt

Journal of neuroinflammation 16:118 PubMed31170999

2019

Reactive microglia and IL1β/IL-1R1-signaling mediate neuroprotection in excitotoxin-damaged mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Levi Todd,Isabella Palazzo,Lilianna Suarez,Xiaoyu Liu,Leo Volkov,Thanh V Hoang,Warren A Campbell,Seth Blackshaw,Ning Quan,Andy J Fischer

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 beta 抗体 - Astrocyte Marker

Anti-S100 beta antibody - Astrocyte Marker

Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise