AB109384

Anti-S100 alpha 抗体 [EPR5251]

Anti-S100 alpha antibody [EPR5251]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal S10A1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 2 publications.

別名を表示する

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (AB109384)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (AB109384)

ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells

Unknown

Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384)

All lanes:

Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution

Lane 1:

Rat kidney tissue lysate at 10 µg

Lane 2:

C2C12 cell lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Human brain lysate at 10 µg

Lane 5:

HT-1080 lysate at 10 µg

Lane 6:

Mouse heart tissue lysate at 10 µg

Predicted band size: 11 kDa

false

Lab

Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384)

Blocking and diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution

All lanes:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 60s

OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (AB109384)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (AB109384)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5251

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.

Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in chemistry 13:1607067 PubMed40552073

2025

HIF1A acts as target of XiHuang Pill in the treatment of papillary thyroid cancer by regulating dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Zhe Yu,Wei-Li Zhu,Hua-Chun Qian,Chun-Jiang Sun

Experimental and therapeutic medicine 25:144 PubMed36911380

2023

Gelatin methacryloyl hydrogel scaffold loaded with activated Schwann cells attenuates apoptosis and promotes functional recovery following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Hao Yu,Peng Yu,Peng Peng,Chao Li,Zhenyang Xiang,Dexiang Ban

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 alpha 抗体 [EPR5251]

Anti-S100 alpha antibody [EPR5251]

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (AB109384)

Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384)

Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384)

OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (AB109384)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise