Anti-Ryanodine receptor 2/RYR-2 抗体 [EPR26288-70]
Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
- 詳細を見る
Be the first to review this product! Submit a review
|
(4 Publications)
Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) is a rabbit monoclonal antibody detecting Ryanodine receptor 2/RYR-2 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
別名を表示する
Ryanodine receptor 2, RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor, RYR2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 (1.068 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : no staining on human skeletal muscle.The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 (1.068 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human cerebrum.The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 (1.068 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : no staining on human liver.The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 (1.068 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human cardiac muscle (PMID : 27987400).The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse heart (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/100 (5.34 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse heart is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/100 (5.34 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat heart is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/2000 (0.267 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on mouse cardiac muscle.The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/100 (5.34 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat liver (PMID : 12395283) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/2000 (0.267 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on rat cardiac muscle (PMID : 27882143).The section was incubated with ab302716 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/100 (5.34 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on mouse liver (PMID : 12395283) is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Ryanodine receptor 2/RYR-2 was immunoprecipitated from 0.35 mg Mouse heart tissue lysate 10 ug with ab302716 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302716 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse heart tissue lysate 10 ug Lane 2 : ab302716 IP in Mouse heart tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab302716 in mouse heart tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes
All lanes:
Immunoprecipitation - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) at 1/1000 dilution
Lane 1:
Mouse heart tissue lysate 10 ug
Lane 2:
ab302716 IP in Mouse heart tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Predicted band size: 564 kDa
Observed band size: 564 kDa
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3 seconds
All lanes:
Western blot - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) at 1/1000 dilution
Lane 1:
HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2:
HEK-293T transfected with human Ryanodine receptor 2 expression vector containi a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 565 kDa
Observed band size: 564 kDa
false
Exposure time: 3s
- WB
Supplier Data
Western blot - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : skeletal muscle, liver (PMID : 23516528, PMID : 9607712) Exposure time : Lanes 1-3 : 15 seconds; Lanes 4-6 : 37 seconds.
All lanes:
Western blot - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) at 1/1000 dilution
Lane 1:
Mouse heart tissue lysate at 20 µg
Lane 2:
Mouse skeletal muscle tissue lysate at 20 µg
Lane 3:
Mouse liver tissue lysate at 20 µg
Lane 4:
Rat heart tissue lysate at 20 µg
Lane 5:
Rat skeletal muscle tissue lysate at 20 µg
Lane 6:
Rat liver tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 565 kDa
Observed band size: 564 kDa
false
関連する標識済み抗体及び組成の異なる製品 (1)
-
Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] - BSA and Azide free
Reactivity data
製品の詳細
What is this antibody validated in?
Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.
What is the molecular weight of Ryanodine receptor 2/RYR-2?
Anti-Ryanodine receptor 2/RYR-2 [EPR26288-70] (ab302716) specifically detects a band for Ryanodine receptor 2/RYR-2 (UniProt: Q92736) at a molecular weight of 565kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR26288-70] also available for your convenience: ab302716, Carrier free - ab302717
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RYR-2 enables the heart muscle cells to contract and relax by supplying calcium ions necessary for muscle contraction. It is part of a larger complex that includes proteins such as FK506-binding protein 12.6 (FKBP12.6) and calsequestrin which help regulate its function. Proper function of this complex is necessary for maintaining the rhythmic contraction and relaxation cycles of the heart. Any disruptions in this function can lead to irregular heartbeats.
Pathways
RYR-2 is significant in cardiac calcium signaling pathways that involve calcium-induced calcium release. Through this pathway it works closely with L-type calcium channels like Cav1.2 which are responsible for the initial influx of calcium that activates RYR-2. This cooperation allows for the amplification of calcium signals needed for strong cardiac muscle contractions. Proper signaling in these pathways is necessary for maintenance of heart rhythm and contractile strength.
製品プロトコール
- Visit the General protocols
- Visit the Troubleshooting
ターゲットの情報
文献 (4)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in nutrition 12:1647017 PubMed41019540
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 26: PubMed40650101
2025
Applications
Unspecified application
Species
Unspecified reactive species
European journal of histochemistry : EJH 68: PubMed39494460
2024
Applications
Unspecified application
Species
Unspecified reactive species
Heliyon 10:e26803 PubMed38434389
2024
Applications
Unspecified application
Species
Unspecified reactive species
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com