Anti-RUNX2 抗体 (ab114133)
Key features and details
- Rabbit polyclonal to RUNX2
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RUNX2 antibody
RUNX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RUNX2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Saos-2 whole cell lysate. ICC: U20S cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114133の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa).
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| 特記事項 |
|---|
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ICC
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. -
組織特異性
Specifically expressed in osteoblasts. -
関連疾患
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. -
配列類似性
Contains 1 Runt domain. -
ドメイン
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. -
翻訳後修飾
Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 472017 Chimpanzee
- Entrez Gene: 860 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q13950 Human
- SwissProt: Q08775 Mouse
- SwissProt: Q9Z2J9 Rat
see all -
別名
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
see all
画像
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Immunocytochemistry - Anti-RUNX2 antibody (ab114133)
ab114133 staining RUNX2 in U2OS cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab114133 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-RUNX2 antibody (ab114133)Anti-RUNX2 antibody (ab114133) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 64 kDa (possible isoform)
Exposure time: 1 minute
The predicted molecular weight of RUNX2 is 56 kDa (SwissProt), however we expect to observe a banding pattern around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2 and 3 of RUNX2. The predicted molecular weights of isoforms 1,2 and 3 are 56, 55 and 54 kDa respectively.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab114133 は 15 報の論文で使用されています。
- Wietecha MS et al. Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes. Matrix Biol 119:19-56 (2023). PubMed: 36914141
- Xu HJ et al. Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH). Mol Biol Rep 50:4769-4779 (2023). PubMed: 37029290
- Yu J et al. Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes. Hum Cell 36:1389-1402 (2023). PubMed: 37269415
- Liu W et al. In situ activation of flexible magnetoelectric membrane enhances bone defect repair. Nat Commun 14:4091 (2023). PubMed: 37429900
- Dong J et al. Pharmacological inhibition of eIF2alpha phosphorylation by integrated stress response inhibitor (ISRIB) ameliorates vascular calcification in rats. Physiol Res 71:379-388 (2022). PubMed: 35616039