Anti-Rubella Virus capsid 抗体 [9B11] (ab34749)
![Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)](https://www.abcam.co.jp/ps/products/34/ab34749/Images/ab34749-47965-anti-rubella-virus-capsid-antibody-9b11-western-blot.jpg "Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)")
Key features and details
- Mouse monoclonal [9B11] to Rubella Virus capsid
- Suitable for: Indirect ELISA, WB
- Reacts with: Rubella virus
- Isotype: IgG1
製品の概要
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製品名
Anti-Rubella Virus capsid antibody [9B11] -
製品の詳細
Mouse monoclonal [9B11] to Rubella Virus capsid -
由来種
Mouse -
アプリケーション
適用あり: Indirect ELISA, WBmore details -
種交差性
交差種: Rubella virus -
免疫原
Recombinant full length protein corresponding to Rubella Virus capsid.
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特記事項
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR120838-1 and GR210838-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
9B11 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34749の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Indirect ELISA |
1/1000 - 1/10000.
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| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 115 kDa.
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| 特記事項 |
|---|
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Indirect ELISA
1/1000 - 1/10000. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 115 kDa. |
ターゲット情報
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関連性
Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It causes German measles ( a mild contagious eruptive disease, capable of producing congenital defects in infants born to mothers infected during the first three months of pregnancy). Rubella virus is an enveloped positive-strand RNA virus. The genome encodes two open reading frames (ORFs): the 5'-proximal ORF encodes viral nonstructural proteins (NSP) that are responsible for viral genome replication, while the 3'-proximal ORF encodes three virion structural proteins (SP), the capsid protein (CP), and the two envelope glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase. -
細胞内局在
Cytoplasmic in host cells concentrated around Golgi region and mitochondrion. -
別名
- Coat protein antibody
- p110 antibody
- rubella capsid antibody
- Structural polyprotein antibody
画像
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Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)All lanes : Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/ml
Lane 1 : Prestained M.Wt markers
Lane 2 : crude yeast cell lysate [mock control]
Lanes 3-4 : irrelevant viral proteins
Lane 5 : recombinant purified rubella capsid protein
Lane 6 : crude lysate of transformed yeast cells expressing rubella capsid protein
Predicted band size: 115 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab34749 は 11 報の論文で使用されています。
- Sakuragi S et al. Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production. Int J Mol Sci 23:N/A (2022). PubMed: 36077193
- Perelygina L et al. Rubella Virus Infected Macrophages and Neutrophils Define Patterns of Granulomatous Inflammation in Inborn and Acquired Errors of Immunity. Front Immunol 12:796065 (2021). PubMed: 35003119
- Pham NTK et al. The Epithelial-to-Mesenchymal Transition-Like Process Induced by TGF-ß1 Enhances Rubella Virus Binding and Infection in A549 Cells via the Smad Pathway. Microorganisms 9:N/A (2021). PubMed: 33806778
- Inoko A et al. Albatross/FBF1 contributes to both centriole duplication and centrosome separation. Genes Cells 23:1023-1042 (2018). PubMed: 30318703
- Trinh QD et al. Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro. Viruses 10:N/A (2018). PubMed: 29300335