Anti-RTKN/Rhotekin 抗体 (ab154954)
Key features and details
- Rabbit polyclonal to RTKN/Rhotekin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RTKN/Rhotekin antibody
RTKN/Rhotekin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RTKN/Rhotekin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Recombinant fragment corresponding to Human RTKN/Rhotekin aa 1-141.
Database link: Q9BST9 -
ポジティブ･コントロール
- WB: 293T, A431, Jurkat, Raji and mouse brain whole cell lysates. A431 cells. ICC/IF: Rhotekin-transfected HeLa cells.
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特記事項
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154954の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 63 kDa. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures. -
組織特異性
Highly expressed in prostate, moderately in kidney, heart, brain, spleen, testis, placenta, small intestine, pancreas, skeletal muscle and peripheral blood leukocytes, and weakly in ovary, colon and thymus. Weakly expressed in all normal cell lines tested. Overexpressed in various cancer cell lines. -
配列類似性
Contains 1 PH domain.
Contains 1 REM (Hr1) repeat. - Information by UniProt
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参照データベース
- Entrez Gene: 6242 Human
- Entrez Gene: 20166 Mouse
- Entrez Gene: 297383 Rat
- Omim: 602288 Human
- SwissProt: Q9BST9 Human
- SwissProt: Q8C6B2 Mouse
- SwissProt: Q6V7V2 Rat
- Unigene: 192854 Human
see all -
別名
- Rhotekin antibody
- Rtkn antibody
- RTKN_HUMAN antibody
- RTKN1 antibody
画像
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Western blot - Anti-RTKN/Rhotekin antibody (ab154954)All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/5000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (ab154954)Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Stained with ab154954 diluted at 1/500 (green). Nuclear staining using Hoechst 33342 (blue).
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Western blot - Anti-RTKN/Rhotekin antibody (ab154954)All lanes : Anti-RTKN/Rhotekin antibody (ab154954) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : human Rhotekin-transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa -
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)Anti-RTKN/Rhotekin antibody (ab154954) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)Anti-RTKN/Rhotekin antibody (ab154954) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg
Predicted band size: 63 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (ab154954)Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling RTKN/Rhotekin with ab154954 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154954 は 3 報の論文で使用されています。
- Wang N & Wang J RTKN2 Inhibits the Growth, Migration, Invasion and Glycolysis of Lung Adenocarcinoma Cells by Inactivating the NF-κB Signalling Pathway. Biochem Genet 61:2135-2148 (2023). PubMed: 36952123
- Zhou J et al. MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN in hepatocellular carcinoma. Oncol Rep 37:1227-1234 (2017). PubMed: 27922690
- Zhang W et al. Inhibition of rhotekin exhibits antitumor effects in lung cancer cells. Oncol Rep 35:2529-34 (2016). WB . PubMed: 26935528