Anti-RSPO1 抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (AB106556)

Immunohistochemical analysis of PFA fixed paraffin embedded Human spleen tissue sections labelling RSPO1 with ab106556, at 2.5 μg/mL.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (AB106556)

Immunohistochemistry of 4% paraformaldehyde-fixed human spleen labeling RSPO1 with 5171 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (AB106556)

Immunohistochemical analysis of PFA fixed paraffin embedded Human heart tissue sections labelling RSPO1 with ab106556, at 2.5 μg/mL.

• WB

Supplier Data

Western blot - Anti-RSPO1 antibody (AB106556)

Lane 1:

Western blot - Anti-RSPO1 antibody (ab106556) at 1 µg/mL

Lane 2:

Western blot - Anti-RSPO1 antibody (ab106556) at 2 µg/mL

All lanes:

Human heart tissue lysate

Predicted band size: 28 kDa

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• WB

Supplier Data

Western blot - Anti-RSPO1 antibody (AB106556)

Lane 1:

Western blot - Anti-RSPO1 antibody (ab106556) at 0.5 µg

Lane 2:

Western blot - Anti-RSPO1 antibody (ab106556) at 1 µg

All lanes:

Human spleen lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 28 kDa

false

• WB

Supplier Data

Western blot - Anti-RSPO1 antibody (AB106556)

All lanes:

Western blot - Anti-RSPO1 antibody (ab106556) at 2 µg

Lane 1:

Daudi cell lysate at 15 µg

Lane 2:

3T3/NIH cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 28 kDa

false

• WB

CiteAb

Western blot - Anti-RSPO1 antibody (AB106556)

RSPO1 western blot using anti-RSPO1 antibody ab106556. Publication image and figure legend from Shi, G. X., Zheng, X. F., et al., 2017, Int J Mol Sci, PubMed 28272338.

ab106556 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab106556 please see the product overview.

Overexpression of Rspo1 enhanced osteogenic differentiation of BMSCs via the Wnt/β-catenin signaling pathway. (A) Western blot and qRT-PCR analysis of Rspo1 after adenovirus infection; (B) qRT-PCR analysis of osteogenic differentiation markers Ocn, Col-1a1 and ALP (Day 3); (C) Relative ALP activities (Day 3); (D) ELISA measurement for supernatant Ocn (Day 3); (E) Representative images of ALP staining (Day 7) and Alizarin red staining (Day 12); (F) qRT-PCR analysis of the Wnt target genes Axin2 and Tcf1; (G) Western blot analysis of the intranuclear level of β-catenin and (H) Topflash dual-luciferase activity assay of BMSCs after 24 h of osteogenic differentiation. The luciferase activities were presented as fold changes normalized to Renilla compared to Con group cells. All data were confirmed by three repeated tests and presented as the mean ± SD, * p < 0.05, ** p < 0.01. p-Values are based on ANOVA and post hoc analysis. ad-GFP : blank adenovirus with green fluorescent protein; ad-Rspo1 : adenovirus with overexpressed Rspo1.

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