Name: Anti-RSK1 p90 (phospho T359) antibody [E238]
Brand: RabMAb
SKU: ab32413
Rating: 5 (8 reviews)

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Western blot - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E238] to RSK1 p90 (phospho T359)
Suitable for: ELISA, WB, IHC-P, IP, Dot blot
Reacts with: Mouse, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品名

Anti-RSK1 p90 (phospho T359) antibody [E238]
RSK1 p90 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [E238] to RSK1 p90 (phospho T359)
由来種

Rabbit
特異性

This antibody is specific for human RSK1 p90 phoshorylated on Threonine 359. 80% identities with RSK3 p90 (pT365+pS369), 75% with RSK2 p90 (pT356+pS360) and 74% identities with RSK6 p90 (pT368+pS372)
アプリケーション

適用あり: ELISA, WB, IHC-P, IP, Dot blotmore details
適用なし: Flow Cyt or ICC/IF
種交差性

交差種: Mouse, Human
交差が予測される動物種: Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: A431 and NIH/3T3 cell lysates. IHC-P: Human cervical carcinoma tissue. IP: NIH/3T3 cell lysate
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

E238
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab32413の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ELISA		Use a concentration of 0 - 1 µg/ml.
WB	(5)	1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P	(2)	Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/100.
Dot blot		1/1000.

特記事項
ELISA Use a concentration of 0 - 1 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/100.
Dot blot 1/1000.

追加情報

Is unsuitable for Flow Cyt or ICC/IF.

機能

Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
配列類似性

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains.
翻訳後修飾

Autophosphorylated on Ser-380, as part of the activation process.
Target information above from: UniProt accession Q15418 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6195 Human
- Entrez Gene: 20111 Mouse
- Entrez Gene: 81771 Rat
- Omim: 601684 Human
- SwissProt: Q15418 Human
- SwissProt: P18653 Mouse
- SwissProt: Q63531 Rat
- Unigene: 149957 Human
- Unigene: 301827 Mouse
- Unigene: 34915 Rat
see all
別名
- 90 kDa ribosomal protein S6 kinase 1 antibody
- dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
- dJ590P13.1 antibody
- EC 2.7.11.1 antibody
- HU 1 antibody
- HU1 antibody
- KS6A1_HUMAN antibody
- MAP kinase activated protein kinase 1a antibody
- MAP kinase-activated protein kinase 1a antibody
- MAPK-activated protein kinase 1a antibody
- MAPKAP kinase 1a antibody
- MAPKAPK-1a antibody
- MAPKAPK1A antibody
- MGC79981 antibody
- Mitogen-activated protein kinase-activated protein kinase 1A antibody
- OTTHUMP00000004113 antibody
- p90 RSK1 antibody
- p90-RSK 1 antibody
- p90rsk antibody
- p90RSK1 antibody
- p90S6K antibody
- pp90RSK1 antibody
- Ribosomal protein S6 kinase 90kD 1 antibody
- Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
- Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
- Ribosomal protein S6 kinase alpha 1 antibody
- Ribosomal protein S6 kinase alpha-1 antibody
- Ribosomal protein S6 kinase polypeptide 1 antibody
- Ribosomal S6 kinase 1 antibody
- RPS6K1 alpha antibody
- rps6ka antibody
- Rps6ka1 antibody
- RSK 1 antibody
- RSK 1 p90 antibody
- RSK antibody
- RSK-1 antibody
- RSK1 antibody
- RSK1p90 antibody
- S6K alpha 1 antibody
- S6K-alpha-1 antibody
see all

Western blot - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

All lanes : Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413) at 1/1000 dilution (purified)

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) grown in serum free media for 4 hour swhole cell lysate
Lane 2 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30minutes whole cell lysate
Lane 3 : NIH/3T3 grown in serum free media for 4 hours, then treated with 20% FBS for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa

Blocking/Diluting Buffer and concentration 5% NFDM/TBST
ELISA - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

ELISA analysis using ab32413 at a range of 0-1000 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L）at 1/2500 dilution. Antigen concentration: 100 ng/ml.

Antigens: Human RSK1 p90 (phospho T359 + S363) peptide, Human RSK1 p90 (phospho T359) peptide, Human RSK1 p90 (phospho S363) peptide, Human RSK1 non-phospho peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

Ab32413, at a 1/100 dilution, staining RSK1 p90 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Left image : Untreated.
Right image : Treated with Phosphatase.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

Purified ab32413 at 1/100 dilution (2µg) immunoprecipitating RSK1 p90 in NIH/3T3 whole cell lysate.
Lane 1 (input): NIH/3T3 (Mouse embryonic fibroblast) serum starved for 4h, then treated with 20% FBS for 30min whole cell lysate 10µg
Lane 2 (+): ab32413 + NIH/3T3 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab171376 in NIH/3T3 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 90 kDa
Lower bands could be cleavage product. (PMID: 29290690)
Dot Blot - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

Dot blot analysis of RSK1 p90 (phospho T359 + S363) phospho peptide (Lane 1), RSK1 p90 phospho T359 phospho peptide (Lane 2), RSK1 p90 phospho S363 phospho peptide (Lane 3) and RSK1 p90 non-phospho peptide (Lane 4) labeling RSK1 p90 (phospho T359 + S363) phospho peptide with purified ab32413 at a dilution of 1/1000 dilution (2.056ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
Western blot - Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413)

All lanes : Anti-RSK1 p90 (phospho T359) antibody [E238] (ab32413) at 1/20000 dilution

Lane 1 : A431 cell lysate untreated.
Lane 2 : A431 cell lysate treated with EGF.

Predicted band size: 90 kDa
Observed band size: 90 kDa
Anti-RSK1 p90 (phospho T359 + S363) antibody [E238] (ab32413)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab32413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32413 は 24 報の論文で使用されています。

Tian J et al. Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial. Nat Med 29:458-466 (2023). PubMed: 36702949
Venkatanarayan A et al. CRAF dimerization with ARAF regulates KRAS-driven tumor growth. Cell Rep 38:110351 (2022). PubMed: 35139374
Rao Q et al. Design, Synthesis, and Antileukemic Evaluation of a Novel Mikanolide Derivative Through the Ras/Raf/MEK/ERK Pathway. Front Pharmacol 13:809551 (2022). PubMed: 35721186
Yu X et al. Rhein inhibits Chlamydia trachomatis infection by regulating pathogen-host cell. Front Public Health 10:1002029 (2022). PubMed: 36238249
Ryan MB et al. KRASG12C-independent feedback activation of wild-type RAS constrains KRASG12C inhibitor efficacy. Cell Rep 39:110993 (2022). PubMed: 35732135

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-8 of 8 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Mouse brain tissue)

Permeabilization

Yes - 0.1% Triton X-100

Specification

Mouse brain tissue

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Hai-Ying Mary Cheng

Verified customer

投稿 Nov 24 2023

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

HeLa cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 06 2021

Application

Western blot

Sample

Rat Cell lysate - whole cell (3611-RF cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

3611-RF cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 06 2021

Application

Western blot

Sample

Monkey Cell lysate - whole cell (COS cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

COS cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 06 2021

Application

Western blot

Sample

Dog Cell lysate - whole cell (MDCK cells)

Gel Running Conditions

Reduced Denaturing (4-15%)

Loading amount

25 µg

Specification

MDCK cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 06 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9

Sample

Human Tissue sections (Human fibromatosis)

Specification

Human fibromatosis

Permeabilization

Yes - Tween-20

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 05 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Antigen retrieval step

Heat mediated

Sample

Mouse Tissue sections (mouse liver)

Specification

mouse liver

Permeabilization

Yes - Tween-20

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 25 2014

Application

Western blot

Loading amount

75 µg

Gel Running Conditions

Reduced Denaturing (10)

Sample

Pig Cell lysate - whole cell (PAE cells)

Specification

PAE cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 07 2014

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

翻訳後修飾

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (24)

レビューと Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-RSK1 p90 (phospho T359) antibody [E238]

Western blot abreview for Anti-RSK1 p90 (phospho T359) antibody [E238]

Western blot abreview for Anti-RSK1 p90 (phospho T359) antibody [E238]

Western blot abreview for Anti-RSK1 p90 (phospho T359) antibody [E238]

Western blot abreview for Anti-RSK1 p90 (phospho T359) antibody [E238]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]

Western blot abreview for Anti-RSK1 p90 (phospho T359 + S363) antibody [E238]