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リコンビナントRabMAb

Anti-RSK1 p90 (phospho S380) 抗体 [E239] (ab32203)

Submit a review Submit a question References (14)
Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
  • Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)
  • Suitable for: WB, ICC/IF, IP, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-RSK1 p90 (phospho S380) antibody [E239]
    RSK1 p90 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E239] to RSK1 p90 (phospho S380)

  • 由来種

    Rabbit

  • 特異性

    The antibody only detects RSK1 phosphorylated on Serine 380.

  • アプリケーション

    適用あり: WB, ICC/IF, IP, Dot blotmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IP: C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes. ICC/IF: A431 cells treated with 100ng/ml EGF for 10 minutes. WB: HeLa starved overnight then treated with 200nM TPA for 4 hours whole cell lysate. PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate. NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32203の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
ICC/IF
1/50.

For the unpurified format use at 1/250 - 1/500 dilution
IP
1/20.

For the unpurified format use at 1/80 dilution
Dot blot
1/1000.
特記事項
WB
1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).
ICC/IF
1/50.

For the unpurified format use at 1/250 - 1/500 dilution
IP
1/20.

For the unpurified format use at 1/80 dilution
Dot blot
1/1000.

ターゲット情報

  • 機能

    Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 2 protein kinase domains.

  • 翻訳後修飾

    Autophosphorylated on Ser-380, as part of the activation process.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 90 kDa ribosomal protein S6 kinase 1 antibody
    • dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody
    • dJ590P13.1 antibody
    • EC 2.7.11.1 antibody
    • HU 1 antibody
    • HU1 antibody
    • KS6A1_HUMAN antibody
    • MAP kinase activated protein kinase 1a antibody
    • MAP kinase-activated protein kinase 1a antibody
    • MAPK-activated protein kinase 1a antibody
    • MAPKAP kinase 1a antibody
    • MAPKAPK-1a antibody
    • MAPKAPK1A antibody
    • MGC79981 antibody
    • Mitogen-activated protein kinase-activated protein kinase 1A antibody
    • OTTHUMP00000004113 antibody
    • p90 RSK1 antibody
    • p90-RSK 1 antibody
    • p90rsk antibody
    • p90RSK1 antibody
    • p90S6K antibody
    • pp90RSK1 antibody
    • Ribosomal protein S6 kinase 90kD 1 antibody
    • Ribosomal protein S6 kinase 90kD polypeptide 1 antibody
    • Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase alpha 1 antibody
    • Ribosomal protein S6 kinase alpha-1 antibody
    • Ribosomal protein S6 kinase polypeptide 1 antibody
    • Ribosomal S6 kinase 1 antibody
    • RPS6K1 alpha antibody
    • rps6ka antibody
    • Rps6ka1 antibody
    • RSK 1 antibody
    • RSK 1 p90 antibody
    • RSK antibody
    • RSK-1 antibody
    • RSK1 antibody
    • RSK1p90 antibody
    • S6K alpha 1 antibody
    • S6K-alpha-1 antibody
    see all

画像

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution

    Lane 1 : Untreated PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) treated with 200ng/ml NGF for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

  • Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Immunoprecipitation - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)

    Purified ab32203 at 1:20 dilution (0.8µg) immunoprecipitating RSK1 p90 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.


    Lane 1 (input): C6 (Rat glial tumor glial cell) whole cell lysate treated with 100nM Calyculin A for 30 minutes 10µg.
    Lane 2 (+): ab32203 + C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32203 in C6 whole cell lysate treated with 100nM Calyculin A for 30 minutes.
    VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:1000 dilution) was used for Western blotting.


    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 90 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 100ng/ml EGF for 10 minutes cells labeling RSK1 p90 with Purified ab32203 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Dot Blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)

    This data was produced using the unpurified format.

    Dot blot analysis of RSK1 p90 (phospho S380) phospho peptide (Lane 1) and RSK1 p90 non-phospho peptide (Lane 2) labeling RSK1 p90 (phospho S380) with ab32203 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight then treated with 200nM TPA for 4 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

  • Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Western blot - Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    All lanes : Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) starved for 24 hours then treated with 100nM Calyculin A for 30 minutes whole cell lysate, and then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Image produced using the purified version.

  • Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)
    Anti-RSK1 p90 (phospho S380) antibody [E239] (ab32203)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (14)

ab32203 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32203 は 14 報の論文で使用されています。

  • Luo Y  et al. Keratin 17 covalently binds to alpha-enolase and exacerbates proliferation of keratinocytes in psoriasis. Int J Biol Sci 19:3395-3411 (2023). PubMed: 37497003
  • Stathopoulou K  et al. Divergent off-target effects of RSK N-terminal and C-terminal kinase inhibitors in cardiac myocytes. Cell Signal 63:109362 (2019). PubMed: 31344438
  • Yang C  et al. CXCL1 stimulates migration and invasion in ER-negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. Int J Oncol 55:684-696 (2019). PubMed: 31322183
  • Shrestha D  et al. Actin Dysfunction Induces Cell Cycle Delay at G2/M with Sustained ERK and RSK Activation in IMR-90 Normal Human Fibroblasts. Mol Cells 41:436-443 (2018). PubMed: 29754473
  • Li FF  et al. Pachymic acid protects H9c2 cardiomyocytes from lipopolysaccharide-induced inflammation and apoptosis by inhibiting the extracellular signal-regulated kinase 1/2 and p38 pathways. Mol Med Rep 12:2807-13 (2015). PubMed: 25936656
View all Publications for this product

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