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AB109002

Anti-RSF1 抗体 [EPR3749(2)]

Anti-RSF1 antibody [EPR3749(2)]

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(10 Publications)

Rabbit Recombinant Monoclonal RSF1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.

別名を表示する

HBXAP, XAP8, RSF1, Remodeling and spacing factor 1, Rsf-1, HBV pX-associated protein 8, Hepatitis B virus X-associated protein, p325 subunit of RSF chromatin-remodeling complex

6 Images
Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with purified ab109002 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human tonsil tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human colon tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Paraffin-embedded human endometrial adenocarcinoma tissue stained for RSF1 with ab109002 (unpurified) at a 1/250 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RSF1 with Purified ab109002 at 1 : 100 (8.7 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-RSF1 antibody [EPR3749(2)] (AB109002)
  • WB

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Western blot - Anti-RSF1 antibody [EPR3749(2)] (AB109002)

All lanes:

Western blot - Anti-RSF1 antibody [EPR3749(2)] (ab109002) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Predicted band size: 163 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-RSF1 antibody [EPR3749(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3749(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p><p><strong>For unpurified use at 1/250- 1/500.</strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/500-1/1000.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/80", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RSF1 also known as Remodeler of Structure of Chromatin 1 is a member of the ATP-dependent chromatin remodeling factors. It has a molecular mass of around 159 kDa. This protein plays a role in altering chromatin architecture by facilitating nucleosome sliding and positioning which is essential for transcriptional regulation. RSF1 expresses in various human tissues particularly in proliferating cells where it assists in regulating gene expression important for cell cycle progression.
Biological function summary

RSF1 participates in cell division and DNA repair processes functioning as part of the RSF complex. This complex along with other chromatin remodelers alters chromatin structure to allow access to DNA. Such mechanisms are critical for allowing correct transcriptional responses during the cell cycle and in response to DNA damage. By modulating nucleosome positioning RSF1 influences the accessibility of transcription factors and other DNA-binding proteins to chromatin.

Pathways

RSF1 involves in the transcriptional regulation and DNA repair pathways. It interacts with proteins like SNF2h within the RSF complex. This interaction is necessary to execute its roles effectively in chromatin remodeling. The involvement of RSF1 in signal transduction pathways also links to the regulation of cell proliferation and survival demonstrating its importance in maintaining cellular homeostasis.

RSF1 associates with cancer development and progression including ovarian and liver carcinoma. Overexpression or mutations within the RSF1 gene can lead to oncogenic transformation supporting tumor growth and metastasis. RSF1's association with other proteins such as key regulators in cancer pathways like p53 indicates its significant role in tumor biology. Understanding its function can provide insights into targeted therapies for cancers involving aberrant RSF1 activity.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Regulatory subunit of the ATP-dependent RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed : 12972596, PubMed : 28801535). Binds to core histones together with SMARCA5, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed : 12972596). Directly stimulates the ATPase activity of SMARCA1 and SMARCA5 in the RSF-1 and RSF-5 ISWI chromatin-remodeling complexes, respectively (PubMed : 28801535). The RSF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the RSF-5 ISWI chromatin-remodeling complex (PubMed : 28801535). The complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain (PubMed : 11788598, PubMed : 11944984).
See full target information RSF1
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:e64 PubMed33693861

2021

aniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Georgios C Stefos,Eszter Szantai,Dimitris Konstantopoulos,Martina Samiotaki,Maria Fousteri

Cancer management and research 12:11845-11855 PubMed33244266

2020

LncRNA NEAT1 Promotes the Progression of Gastric Cancer Through Modifying the miR-1224-5p/RSF1 Signaling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Luoluo Yang,Min Wang,Ping He

Cancer biotherapy & radiopharmaceuticals 36:202-210 PubMed33156701

2020

lncRNA-PRLB Confers Paclitaxel Resistance of Ovarian Cancer Cells by Regulating RSF1/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzi Zhao,Li Hong

Acta oncologica (Stockholm, Sweden) 59:268-273 PubMed31687881

2019

High RSF1 protein expression is an independent prognostic feature in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Doris Höflmayer,Moslim Hamuda,Cornelia Schroeder,Claudia Hube-Magg,Ronald Simon,Cosima Göbel,Andrea Hinsch,Sören Weidemann,Katharina Möller,Jacob R Izbicki,Frank Jacobsen,Tim Mandelkow,Niclas C Blessin,Florian Lutz,Florian Viehweger,Guido Sauter,Eike Burandt,Patrick Lebok,Maximillian Lennartz,Christoph Fraune,Sarah Minner,Sarah Bonk,Hartwig Huland,Markus Graefen,Thorsten Schlomm,Franziska Büscheck

Nature communications 9:3848 PubMed30242288

2018

The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation.

Applications

Unspecified application

Species

Unspecified reactive species

Ho-Soo Lee,Zhonghui Lin,Sunyoung Chae,Young-Suk Yoo,Byung-Gyu Kim,Youngsoo Lee,Jared L Johnson,You-Sun Kim,Lewis C Cantley,Chang-Woo Lee,Hongtao Yu,Hyeseong Cho

Oncology letters 16:4279-4284 PubMed30214561

2018

Effects of RSF-1 on proliferation and apoptosis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhui Liu,Junda Gai,Lin Fu,Xiuwei Zhang,Enhua Wang,Qingchang Li

Molecules and cells 41:127-133 PubMed29385673

2018

Post-Translational Regulation of the RSF1 Chromatin Remodeler under DNA Damage.

Applications

WB

Species

Unspecified reactive species

Sunwoo Min,Yong Won Choi,Hansol Yun,Sujin Jo,Jae-Hoon Ji,Hyeseong Cho

Oncology reports 38:2727-2734 PubMed29048677

2017

MicroRNA-154 as a prognostic factor in bladder cancer inhibits cellular malignancy by targeting RSF1 and RUNX2.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhao,Zhigang Ji,Yi Xie,Guanghua Liu,Hanzhong Li

Nature communications 6:7904 PubMed26259146

2015

The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores.

Applications

ICC

Species

Unspecified reactive species

Ho-Soo Lee,Yong-Yea Park,Mi-Young Cho,Sunyoung Chae,Young-Suk Yoo,Myung-Hee Kwon,Chang-Woo Lee,Hyeseong Cho

International journal of clinical and experimental 7:5595-608 PubMed25337201

2014

Overexpression of Rsf-1 correlates with pathological type, p53 status and survival in primary breast cancer.

Applications

IHC

Species

Human

Jie Ren,Qiu-Chen Chen,Feng Jin,Hui-Zhe Wu,Miao He,Lin Zhao,Zhao-Jin Yu,Wei-Fan Yao,Xiao-Yi Mi,En-Hua Wang,Min-Jie Wei
View all publications
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Abcam product promise

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