Anti-RRP9 抗体 (ab168845)
Key features and details
- Rabbit polyclonal to RRP9
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RRP9 antibody -
製品の詳細
Rabbit polyclonal to RRP9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human RRP9 (NP_004695.1).
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ポジティブ･コントロール
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab168845 was affinity purified using an epitope specific to RRP9 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168845の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 52 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 52 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA. -
配列類似性
Belongs to the WD repeat RRP9 family.
Contains 7 WD repeats. -
ドメイン
The WD domains are required for nucleolar localization and U3 small nucleolar RNAs binding. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 460407 Chimpanzee
- Entrez Gene: 101145893 Gorilla
- Entrez Gene: 9136 Human
- Entrez Gene: 100439547 Orangutan
- Entrez Gene: 699421 Rhesus monkey
- SwissProt: O43818 Human
- Unigene: 153768 Human
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別名
- Ribosomal RNA processing 9 small subunit (SSU) processome component homolog antibody
- RNA U3 small nucleolar interacting protein 2 antibody
- RNU3IP2 antibody
see all
画像
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Western blot - Anti-RRP9 antibody (ab168845)All lanes : Anti-RRP9 antibody (ab168845) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-RRP9 antibody (ab168845)Detection of RRP9 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168845 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab168845 は 2 報の論文で使用されています。
- Du MG et al. Neddylation modification of the U3 snoRNA-binding protein RRP9 by Smurf1 promotes tumorigenesis. J Biol Chem 297:101307 (2021). PubMed: 34662580
- Cai Y et al. Broad Susceptibility of Nucleolar Proteins and Autoantigens to Complement C1 Protease Degradation. J Immunol 199:3981-3990 (2017). PubMed: 29070672