AB168845

Anti-RRP9 抗体

Name: Anti-RRP9 抗体 (ab168845) | Abcam
Brand: Abcam Limited
SKU: AB168845
Price: 71000 JPY
Availability: InStock

Anti-RRP9 antibody

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(4 Publications)

Rabbit Polyclonal RRP9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RRP9 aa 150-250.

別名を表示する

RNU3IP2, U355K, RRP9, U3 small nucleolar RNA-interacting protein 2, RRP9 homolog, U3 small nucleolar ribonucleoprotein-associated 55 kDa protein, U3 snoRNP-associated 55 kDa protein, U3-55K

2 Images

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Immunoprecipitation - Anti-RRP9 antibody (AB168845)

Detection of RRP9 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168845 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RRP9 antibody (ab168845)

Predicted band size: 52 kDa

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Unknown

Western blot - Anti-RRP9 antibody (AB168845)

All lanes:

Western blot - Anti-RRP9 antibody (ab168845) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 52 kDa

true

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human RRP9 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O43818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab168845 was affinity purified using an epitope specific to RRP9 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RRP9 also known as Ribosomal RNA Processing 9 is a protein that plays a mechanical role in ribosome biogenesis. It has a molecular mass of approximately 74 kDa. The protein is mainly expressed in the nucleolus the site where ribosomal RNA synthesis and initial steps of ribosome assembly occur. RRP9 contains several conserved motifs that enable it to bind RNA and participate in various processing events essential for this function.

Biological function summary

The protein functions as a component of the small subunit (SSU) processome a large ribonucleoprotein complex. RRP9 contributes to the early pre-rRNA processing steps that produce the small subunit of the ribosome. The SSU processome carries out essential modifications and cleavages necessary for the maturation and functionality of ribosomes. Therefore RRP9 is critical to ensure accurate production of ribosomal subunits.

Pathways

RRP9 is involved in ribosome biogenesis and RNA processing pathways. Through its role in the SSU processome it connects with other proteins such as UTP-C and MPP10 which coordinate these pathways. Ribosome biogenesis is fundamental for protein synthesis and cell proliferation establishing RRP9's importance in these cellular processes. Proper coordination of these pathways is necessary for maintaining cellular homeostasis and function.

RRP9 relates to ribosomopathies conditions caused by defects in ribosomal function. Mutations or dysregulation of RRP9 can result in impaired ribosome assembly contributing to disorders like Diamond-Blackfan anemia. In this disorder RRP9's interaction with other ribosomal proteins like RPS19 becomes disrupted leading to defective erythropoiesis. Additionally abnormal RRP9 expression can be linked to cancer development where increased ribosome production is a common feature.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA (pre-rRNA) (PubMed : 26867678). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information RRP9

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:7436 PubMed38548901

2024

CRISPR/Cas9 targeting of passenger single nucleotide variants in haploinsufficient or essential genes expands cancer therapy prospects.

Applications

Unspecified application

Species

Unspecified reactive species

Hakhyun Kim,Jang Hee Han,Hyosil Kim,Minjee Kim,Seung-Il Jo,NaKyoung Lee,Seungbin Cha,Myung Joon Oh,GaWon Choi,Hyun Seok Kim

Clinical and translational medicine 13:e1235 PubMed37186134

2023

Therapeutic targeting of CNBP phase separation inhibits ribosome biogenesis and neuroblastoma progression via modulating SWI/SNF complex activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anpei Hu,Guo Chen,Banghe Bao,Yanhua Guo,Dan Li,Xiaojing Wang,Jianqun Wang,Qilan Li,Yi Zhou,Haiyang Gao,Jiyu Song,Xinyi Du,Liduan Zheng,Qiangsong Tong

The Journal of biological chemistry 297:101307 PubMed34662580

2021

Neddylation modification of the U3 snoRNA-binding protein RRP9 by Smurf1 promotes tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ge Du,Fan Liu,Yan Chang,Shuai Tong,Wei Liu,Yu-Jiao Chen,Ping Xie

Journal of immunology (Baltimore, Md. : 1950) 199:3981-3990 PubMed29070672

2017

Broad Susceptibility of Nucleolar Proteins and Autoantigens to Complement C1 Protease Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yitian Cai,Seng Yin Kelly Wee,Junjie Chen,Boon Heng Dennis Teo,Yee Leng Carol Ng,Khai Pang Leong,Jinhua Lu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RRP9 抗体

Anti-RRP9 antibody

Immunoprecipitation - Anti-RRP9 antibody (AB168845)

Western blot - Anti-RRP9 antibody (AB168845)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise