Anti-RRP4 抗体 [EPR13606(B)] - C-terminal (ab185235)
![Western blot - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.co.jp/ps/products/185/ab185235/Images/ab185235-218609-anti-rrp4-antibody-epr13606b-c-terminal-western-blot.jpg "Western blot - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RRP4 antibody [EPR13606(B)] - C-terminal
RRP4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HepG2, HeLa, 293 and Jurkat cell lysates; Human stomach tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13606(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185235の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/180.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
The exosome, present in both the nucleus and cytoplasm of all eukaryotic cells, is a complex of 3'-5' exoribonucleases containing at least nine core components. Recently, it has been demonstrated, mainly by analyses in yeast, that the nuclear exosome is essential for rRNA processing and sn(o)RNA biogenesis. Furthermore, it is involved in the degradation of improperly processed mRNAs. The cytoplasmic exosome participates in normal mRNA turnover and in the degradation of inherently instable mRNAs that contain AU-rich elements. Therefore, the exosome plays a key role in RNA metabolism. -
細胞内局在
Cytoplasmic, Exosome and Nuclear -
参照データベース
- Entrez Gene: 23404 Human
- Entrez Gene: 227715 Mouse
- Entrez Gene: 366017 Rat
- GenBank: NP_055100.2 Human
- Omim: 602238 Human
- SwissProt: Q13868 Human
- SwissProt: Q8VBV3 Mouse
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別名
- EXOSC2 antibody
- Exosome complex exonuclease RRP4 antibody
- Exosome component 2 antibody
see all
画像
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Western blot - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)All lanes : Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.co.jp/ps/products/185/ab185235/Images/ab185235-218608-anti-rrp4-antibody-epr13606b-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RRP4 with ab185235 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.co.jp/ps/products/185/ab185235/Images/ab185235-218607-anti-rrp4-antibody-epr13606b-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling RRP4 with ab185235at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.co.jp/ps/products/185/ab185235/Images/ab185235-4-benefits-of-recombinant-antibodies.png)
Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab185235 は論文での使用が確認できていません。