Name: Anti-RRM2 antibody [1E1]
SKU: ab57653
Rating: 4 (3 reviews)

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Western blot - Anti-RRM2 antibody [1E1] (ab57653)

Key features and details

Mouse monoclonal [1E1] to RRM2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG1

製品名

Anti-RRM2 antibody [1E1]
RRM2 一次抗体製品一覧
製品の詳細

Mouse monoclonal [1E1] to RRM2
由来種

Mouse
アプリケーション

適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
種交差性

交差種: Human
免疫原

Recombinant fragment, corresponding to amino acids 1-111 of Human RRM2
特記事項

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファー

pH: 7.40
Constituent: 100% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

1E1
アイソタイプ

IgG1
軽鎖の種類

kappa
研究分野

Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Recombinant Human RRM2 protein (ab99137)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab57653の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.
Flow Cyt		Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

機能

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
パスウェイ

Genetic information processing; DNA replication.
配列類似性

Belongs to the ribonucleoside diphosphate reductase small chain family.
翻訳後修飾

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession P31350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6241 Human
- Omim: 180390 Human
- SwissProt: P31350 Human
- Unigene: 226390 Human
別名
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
- R2 antibody
- reductase M2 polypeptide variant 1 antibody
- reductase M2 polypeptide variant 2 antibody
- reductase M2 polypeptide variant 3a antibody
- reductase M2 polypeptide variant 3b antibody
- reductase M2 polypeptide variant 3c antibody
- reductase M2 polypeptide variant 3d antibody
- Ribonucleoside-diphosphate reductase subunit M2 antibody
- Ribonucleotide reductase M2 antibody
- Ribonucleotide reductase M2 polypeptide antibody
- Ribonucleotide reductase M2 subunit antibody
- ribonucleotide reductase protein r2 class I antibody
- Ribonucleotide reductase regulatory subunit M2 antibody
- Ribonucleotide reductase small chain antibody
- Ribonucleotide reductase small subunit antibody
- Ribonucleotide reductase, R2 subunit antibody
- RIR2_HUMAN antibody
- RR2 antibody
- RR2M antibody
- RRM2 antibody
see all

Western blot - Anti-RRM2 antibody [1E1] (ab57653)

Anti-RRM2 antibody [1E1] (ab57653) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 45 kDa

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RRM2 (green) using ab57653 at 10 µg/ml in ICC/IF.

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRM2 antibody [1E1] (ab57653)

RRM2 antibody (ab57653) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach.

This image was generated using the ascites version of the product.
Western blot - Anti-RRM2 antibody [1E1] (ab57653)

RRM2 antibody (ab57653) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.
Immunocytochemistry/ Immunofluorescence - Anti-RRM2 antibody [1E1] (ab57653)

ICC/IF image of ab57653 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57653, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
Flow Cytometry - Anti-RRM2 antibody [1E1] (ab57653)

Overlay histogram showing HepG2 cells stained with ab57653 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57653, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab57653 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab57653 は 38 報の論文で使用されています。

Song X et al. Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma. Front Oncol 12:810526 (2022). PubMed: 35311093
Liu Y et al. MiR-200c-3p and miR-485-5p overexpression elevates cisplatin sensitivity and suppresses the malignant phenotypes of non-small cell lung cancer cells through targeting RRM2. Thorac Cancer 13:1974-1985 (2022). PubMed: 35599447
Fu K et al. LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis. Orphanet J Rare Dis 17:119 (2022). PubMed: 35248107
Cao X et al. MiR-202-3p inhibits the proliferation and metastasis of lung adenocarcinoma cells by targeting RRM2. Ann Transl Med 10:1374 (2022). PubMed: 36660663
Bothou C et al. Novel Insights into the Molecular Regulation of Ribonucleotide Reductase in Adrenocortical Carcinoma Treatment. Cancers (Basel) 13:N/A (2021). PubMed: 34439352

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (hiPSC)

Gel Running Conditions

Reduced Denaturing

Loading amount

15 µg

Specification

hiPSC

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Jason Halliwell

Verified customer

投稿 May 14 2019

Application

Western blot

Loading amount

10000 cells

Gel Running Conditions

Reduced Denaturing (8%)

Sample

Human Cell lysate - whole cell (293T)

Specification

293T

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 20 2014

Application

Western blot

Loading amount

10000 cells

Gel Running Conditions

Reduced Denaturing (8%)

Sample

African Green Monkey Cell lysate - whole cell (COS-7 - AGM kidney tissue fibroblast like line)

Specification

COS-7 - AGM kidney tissue fibroblast like line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 19 2014

Question

Bonjour, à la suite de votre e-mail, je serais intéressée pour avoir une offre de test d'anticorps Abcam pour les ac suivant: anti-RPS23 (ab57644) anti Pumilio 2 (ab57049) ce sont des anticorps monoclonaux, et la séquence fournie sur la fiche technique est homologue à 97% et 88% respectivement avec les sequences d'oursin. Je n'ai pas vu de polyclonaux, mais peut-etre ai-je mal cherché. Un troisieme anticorps monoclonal m'interesse, anti-RRM2 (ab57653), mais seulement un fragment de 23aa est tres conservé sur les 92 aa ayant servi d'antigène. Qu'en pensez-vous? Merci de votre aide par avance

Abcam community

Verified customer

Asked on Jan 05 2012

Answer

Merci de nous avoir contactés. Nous serons ravis de vous envoyer un code de test pour des anticorps anti-RPS23, anti-pumilio2 et anti-RRM2. Avant l'édition de ces codes, je vous invite à consulter les listes des six anti-pumilio2 et des quatre anti-RRM2 que nous avons à notre catalogue. anti-Pumilio 2 : https://www.abcam.com/index.html?pageconfig=searchresults&search=Pumilio 2&pt=1&sk=targ&sv=Pumilio 2&sn=Pumilio 2&l=1&fViewMore=1 anti-RRM2 : https://www.abcam.com/index.html?pageconfig=searchresults&search=RRM2&pt=1&sk=targ&sv=RRM2&sn=RRM2&l=1&fViewMore=1 L'anti-RPS23 polyclonal alternatif à ab57644 est le ab110399 pour lequel je suis en contact avec le laboratoire afin d'obtenir la séquence exacte de l'immunogène. Le lien vers la fiche technique est : www.abcam.com/ab110399 J'espère que ces informations vous seront utiles. Je vous contacterai dès que j'aurai reçu une réponse du laboratoire qui produit l'anti-RPS23 ab110399.

Abcam Scientific Support

Answered on Jan 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

軽鎖の種類

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

パスウェイ

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (38)

レビューと Q&A

Western blot abreview for Anti-RRM2 antibody [1E1]

Western blot abreview for Anti-RRM2 antibody

Western blot abreview for Anti-RRM2 antibody