Anti-RPS6 抗体 - Ribosome Marker (ab70227)
Key features and details
- Rabbit polyclonal to RPS6 - Ribosome Marker
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPS6 antibody - Ribosome Marker
RPS6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPS6 - Ribosome Marker -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Reptile, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 100 and 150 of human RPS6. ( NP_001001.2).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
配列類似性
Belongs to the ribosomal protein S6e family. -
翻訳後修飾
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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参照データベース
- Entrez Gene: 396148 Chicken
- Entrez Gene: 787914 Cow
- Entrez Gene: 474722 Dog
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 100038023 Pig
- Entrez Gene: 100338367 Rabbit
see all -
別名
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
画像
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Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227)This image is courtesy of an anonymous AbreviewAnti-RPS6 antibody - Ribosome Marker (ab70227) at 1/1000 dilution + Caenorhabditis elegans whole adult tissue lysate at 200 µg
Secondary
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible non-specific binding), 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
Exposure time: 40 seconds
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Western blot - Anti-RPS6 antibody - Ribosome Marker (ab70227)All lanes : Anti-RPS6 antibody - Ribosome Marker (ab70227) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-RPS6 antibody - Ribosome Marker (ab70227)Detection of Human RPS6 by Immunoprecipitation. Samples: A) Whole cell lysate from HeLa cells (1 mg/IP; 1/4 of IP loaded) using affinity purified ab70227 at 3 µg/mg lysate for IP and at 0.04 µg/ml for subequent WB detection. Second lane represents IgG IP control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab70227 は 12 報の論文で使用されています。
- Zhang ZJ et al. EEF1A2 accelerates the protein translation of chemokine in rat myocardial cells induced by ischemia-reperfusion. Heliyon 9:e15305 (2023). PubMed: 37101626
- Hattori K et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
- Liu Z et al. SNORA23 inhibits HCC tumorigenesis by impairing the 2'-O-ribose methylation level of 28S rRNA. Cancer Biol Med 19:104-19 (2021). PubMed: 33710804
- Segreto R et al. The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride. PLoS One 16:e0262180 (2021). PubMed: 34972198
- Matsumoto N et al. PP1C and PP2A are p70S6K Phosphatases Whose Inhibition Ameliorates HLD12-Associated Inhibition of Oligodendroglial Cell Morphological Differentiation. Biomedicines 8:N/A (2020). PubMed: 32316234