AB175219

Anti-RPS12 抗体 [EPR10963]

Anti-RPS12 antibody [EPR10963]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal RPS12 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

別名を表示する

Small ribosomal subunit protein eS12, 40S ribosomal protein S12, RPS12

4 Images

Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (AB175219)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (AB175219)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (AB175219)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (AB175219)

Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (AB175219)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (ab175219)

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-RPS12 antibody [EPR10963] (AB175219)

All lanes:

Western blot - Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Lane 6:

RAW 264.7 cell lysate at 10 µg

Lane 7:

NIH/3T3 cell lysate at 10 µg

Predicted band size: 15 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10963

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IP, ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPS12 also known as Ribosomal Protein S12 is a component of the 40S ribosomal subunit. It has a molecular mass of approximately 14 kDa. This protein plays an important role in the synthesis of proteins by helping to translate messenger RNA into polypeptides. It is expressed widely in human tissues indicating its fundamental role in cellular function. RPS12 is an integral part of ribosome structure and functionality contributing to the assembly and stability of the ribosomes.

Biological function summary

Ribosomal Protein S12 is involved in forming the small ribosomal subunit and it is a part of the ribonucleoprotein complex that is responsible for decoding genetic information. This complex ensures the accurate translation of genetic sequences which is essential for proper cell division and growth. The function of RPS12 extends to maintaining the fidelity of translation ensuring that amino acids are added correctly during protein synthesis.

Pathways

The role of Ribosomal Protein S12 is significant in the mRNA translation pathway affecting gene expression and protein production. It is directly linked to the regulation of the translation machinery. RPS12 is associated with various other ribosomal proteins such as RPS6 and RPL5 to form a coordinated network that influences protein synthesis and cellular responses to environmental changes. Its interaction with these proteins underpins its participation in the global control of protein synthesis.

Altered expression or mutations in Ribosomal Protein S12 have connections with certain ribosomopathies. Disorders like Diamond-Blackfan anemia relate to dysfunctions within ribosomal components including RPS12. Moreover aberrations in ribosomal proteins including RPS12 can predispose individuals to cancer by disrupting normal cell growth control. Understanding how RPS12 interacts with proteins involved in these diseases can provide insights into potential therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797). Subunit of the 40S ribosomal complex (By similarity).

See full target information RPS12

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:731896 PubMed34485161

2021

Transcriptome Profiling Reveals B-Lineage Cells Contribute to the Poor Prognosis and Metastasis of Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fafen Yang,Jingjie Zhao,Xiuzhuang Luo,Tong Li,Zechen Wang,Qiuju Wei,Heming Lu,Yiliang Meng,Kai Cai,Liuying Lu,Yushi Lu,Lifen Chen,Suren Rao Sooranna,Linxue Luo,Jian Song,Lingzhang Meng

RNA (New York, N.Y.) 26:361-371 PubMed31911497

2020

Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Brumwell,Leslie Fell,Lindsay Obress,James Uniacke

iScience 3:87-101 PubMed30428332

2018

mTORC1 Negatively Regulates the Replication of Classical Swine Fever Virus Through Autophagy and IRES-Dependent Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Luo,Li Zhang,Feng Wei,Qiang Fang,Fei Bao,Shijiang Mi,Nan Li,Chengming Wang,Yan Liu,Changchun Tu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPS12 抗体 [EPR10963]

Anti-RPS12 antibody [EPR10963]

Flow Cytometry (Intracellular) - Anti-RPS12 antibody [EPR10963] (AB175219)

Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (AB175219)

Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (AB175219)

Western blot - Anti-RPS12 antibody [EPR10963] (AB175219)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise