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AB88872

Anti-RPLP0 抗体

Anti-RPLP0 antibody

3

(2 Reviews)

|

(6 Publications)

Mouse Polyclonal RPLP0 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human 60S acidic ribosomal protein P0.

別名を表示する

Large ribosomal subunit protein uL10, 60S acidic ribosomal protein P0, 60S ribosomal protein L10E, RPLP0

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody (AB88872)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPLP0 antibody (AB88872)

ab88872 at 10μg/ml staining RPLP0 in HeLa cells by Immunofluorescence.

Western blot - Anti-RPLP0 antibody (AB88872)
  • WB

Unknown

Western blot - Anti-RPLP0 antibody (AB88872)

All lanes:

Western blot - Anti-RPLP0 antibody (ab88872) at 1 µg/mL

Lane 1:

RPLP0 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-RPLP0 antibody (AB88872)
  • WB

Unknown

Western blot - Anti-RPLP0 antibody (AB88872)

All lanes:

Western blot - Anti-RPLP0 antibody (ab88872) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Key facts

宿主種

Mouse

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human 60S acidic ribosomal protein P0.

P05388

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPLP0 also known as 60S acidic ribosomal protein P0 is an integral component of the ribosomal machinery. It acts as a scaffold within the large 60S subunit of the ribosome which plays a fundamental role in protein synthesis. The protein has an approximate mass of 34.4 kDa. RPLP0 is ubiquitously expressed across various tissues indicating its essential role in cellular biology. Its expression is well-documented in highly proliferative tissues and organs reflecting the ribosome's central role in maintaining cellular growth and division.
Biological function summary

RPLP0 participates actively in ribosome structure and function. It forms part of the ribosomal stalk together with the P1 and P2 proteins facilitating interactions with GTPase translation factors during protein synthesis. This ribosomal complex is important for elongation phase of protein translation where it actively assists in the accurate and efficient elongation of polypeptide chains. By participating in the ribosome's structural framework RPLP0 drives the translation process influencing protein output in cells. This makes it essential for overall protein homeostasis.

Pathways

RPLP0 is deeply embedded within the processes of translation and ribosome biogenesis. It associates with the major pathway of ribosome assembly wherein new ribosomes are synthesized and configured. It functions alongside other ribosomal proteins like RPLP1 and RPLP2 which work together to maintain efficient protein synthesis. The interaction among these proteins within the translation pathway highlights the integrated nature of RPLP0 in facilitating cellular protein production.

RPLP0 has notable implications in cancer and Diamond-Blackfan anemia (DBA). Aberrations in ribosomal proteins including RPLP0 have been linked to oncogenesis where disrupted protein synthesis contributes to uncontrolled cellular proliferation. Additionally alterations in RPLP0 have associations with DBA a ribosomopathy characterized by defective red blood cell production. Within these disease contexts RPLP0's interaction with other ribosomal proteins like RPL11 can influence disease progression and cellular anomalies seen in such conditions.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
See full target information 60S acidic ribosomal protein P0

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36361985

2022

High-Resolution Crystal Structure of Muscle Phosphoglycerate Mutase Provides Insight into Its Nuclear Import and Role.

Applications

Unspecified application

Species

Unspecified reactive species

Janusz Wiśniewski,Jakub Barciszewski,Jakub Turlik,Karolina Baran,Przemysław Duda,Mariusz Jaskolski,Dariusz Rakus

eLife 7: PubMed30095066

2018

Efficient analysis of mammalian polysomes in cells and tissues using Ribo Mega-SEC.

Applications

Unspecified application

Species

Unspecified reactive species

Harunori Yoshikawa,Mark Larance,Dylan J Harney,Ramasubramanian Sundaramoorthy,Tony Ly,Tom Owen-Hughes,Angus I Lamond

Nature communications 7:12156 PubMed27402251

2016

ERRα mediates metabolic adaptations driving lapatinib resistance in breast cancer.

Applications

WB

Species

Human

Geneviève Deblois,Harvey W Smith,Ingrid S Tam,Simon-Pierre Gravel,Maxime Caron,Paul Savage,David P Labbé,Louis R Bégin,Michel L Tremblay,Morag Park,Guillaume Bourque,Julie St-Pierre,William J Muller,Vincent Giguère

PloS one 9:e91465 PubMed24658146

2014

Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin.

Applications

ICC/IF

Species

Human

Mohamed S Taha,Kazem Nouri,Lech G Milroy,Jens M Moll,Christian Herrmann,Luc Brunsveld,Roland P Piekorz,Mohammad R Ahmadian

Proceedings of the National Academy of Sciences of 110:17975-80 PubMed24127579

2013

Loss of estrogen-related receptor α promotes hepatocarcinogenesis development via metabolic and inflammatory disturbances.

Applications

WB

Species

Mouse

Eui-Ju Hong,Marie-Pier Levasseur,Catherine R Dufour,Marie-Claude Perry,Vincent Giguère

PloS one 5:e11829 PubMed20676407

2010

HSV usurps eukaryotic initiation factor 3 subunit M for viral protein translation: novel prevention target.

Applications

WB

Species

Human

Natalia Cheshenko,Janie B Trepanier,Theodore J Segarra,A Oveta Fuller,Betsy C Herold
View all publications

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