AB249490

Anti-RPL8 抗体 [EPR11630] - BSA and Azide free

Anti-RPL8 antibody [EPR11630] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RPL8 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Large ribosomal subunit protein uL2, 60S ribosomal protein L8, RPL8

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2cells labeling RPL8 with ab169538 at 1/250.

Unknown

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.Immunoprecipitation. ab169538 at 1/10000 staining RPL8 in Jurkat cell lysate immunoprecipitated using ab169538 at 1/10. Predicted band size : 28 kDa.

All lanes:

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] (<a href='/products/primary-antibodies/rpl8-antibody-epr11630-ab169538'>ab169538</a>)

Predicted band size: 28 kDa

false

Unknown

Western blot - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

This data was developed using ab169538, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RPL8 antibody [EPR11630] (<a href='/products/primary-antibodies/rpl8-antibody-epr11630-ab169538'>ab169538</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

Molt4 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11630

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab249490 is the carrier-free version of ab169538.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPL8 also known as Ribosomal Protein L8 is a component of the 60S subunit of the ribosome playing a critical role in protein synthesis. It has a mass of approximately 26.1 kDa. RPL8 is expressed in a wide range of tissues including the liver kidney and brain reflecting its essential role in cellular function. This protein contributes directly to the structure and function of the ribosome facilitating the translation of mRNA into polypeptides.

Biological function summary

RPL8 serves as an integral part of the ribosomal machinery ensuring effective protein synthesis within cells. It is part of the large ribosomal subunit complex working alongside other ribosomal proteins to form a functional ribosome. This role is paramount for maintaining cellular homeostasis and supporting growth and proliferation by enabling balanced protein production.

Pathways

RPL8 is key within several fundamental biological pathways including the translation pathway and ribosome biogenesis. In ribosome biogenesis it interacts with proteins such as RPL5 and RPL11 contributing to the assembly and maturation of ribosomal subunits. This interaction facilitates the complex process of protein synthesis ensuring the correct translation of genetic information into functional proteins.

Abnormalities in RPL8 expression or function have been linked to certain cancers and Diamond-Blackfan anemia. In cancer dysfunction in ribosomal protein balance including RPL8 can lead to unchecked cellular proliferation. In Diamond-Blackfan anemia defects associated with RPL8 and other ribosomal proteins like RPS19 cause ineffective erythropoiesis leading to the anemia typically observed in this condition. Understanding these connections helps guide potential therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL8

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL8 抗体 [EPR11630] - BSA and Azide free

Anti-RPL8 antibody [EPR11630] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

Immunoprecipitation - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

Western blot - Anti-RPL8 antibody [EPR11630] - BSA and Azide free (AB249490)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise