Anti-RPL7A 抗体 (ab70753)
Key features and details
- Rabbit polyclonal to RPL7A
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL7A antibody
RPL7A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL7A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to Human RPL7A (C terminal).
Database link: P62424 -
ポジティブ･コントロール
- IHC-P: human colon carcinoma, mouse renal cell carcinoma. WB: HeLa, MCF7, HEK-293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70753の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
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| 特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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関連疾患
Note=Chromosomal recombination involving RPL7A activates the receptor kinase domain of the TRK oncogene. -
配列類似性
Belongs to the ribosomal protein L7Ae family. - Information by UniProt
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参照データベース
- Entrez Gene: 417158 Chicken
- Entrez Gene: 513128 Cow
- Entrez Gene: 6130 Human
- Entrez Gene: 27176 Mouse
- Entrez Gene: 296596 Rat
- Entrez Gene: 443912 Xenopus laevis
- Entrez Gene: 447981 Xenopus tropicalis
- Entrez Gene: 337010 Zebrafish
see all -
別名
- 60S ribosomal protein L7a antibody
- L7A antibody
- MGC103146 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL7A antibody (ab70753)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling RPL7A with ab70753 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-RPL7A antibody (ab70753)All lanes : Anti-RPL7A antibody (ab70753) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa whole cell lysate at 4 µg
Lane 3 : MCF7 whole cell lysate at 20 µg
Lane 4 : 293T whole cell lysate at 20 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab70753 は 2 報の論文で使用されています。
- Sahoo MR et al. Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC. EMBO Rep 18:241-263 (2017). PubMed: 28039207
- Yang PY et al. Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities. J Am Chem Soc 132:656-66 (2010). PubMed: 20028024