Anti-RPL4 抗体 (ab154907)
Key features and details
- Rabbit polyclonal to RPL4
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL4 antibody
RPL4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow
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免疫原
Recombinant fragment, corresponding to a region within amino acids 60-340 of Human RPL4 (Uniprot ID: P36578).
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ポジティブ･コントロール
- WB: Raji, K562 and NCI-H929 whole cell lysates; IHC-P: Human U87 xenograft, mouse colon and pancreas tissue; IF: HepG2 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154907の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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配列類似性
Belongs to the ribosomal protein L4P family. - Information by UniProt
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参照データベース
- Entrez Gene: 415551 Chicken
- Entrez Gene: 6124 Human
- Entrez Gene: 67891 Mouse
- Entrez Gene: 64302 Rat
- Omim: 180479 Human
- SwissProt: Q58DW0 Cow
- SwissProt: P36578 Human
- SwissProt: Q9D8E6 Mouse
see all -
別名
- 60S ribosomal protein L1 antibody
- 60S ribosomal protein L4 antibody
- L4 antibody
see all
画像
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Western blot - Anti-RPL4 antibody (ab154907)All lanes : Anti-RPL4 antibody (ab154907) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 48 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded human U87 xenograft tissue staining RPL4 with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry - Anti-RPL4 antibody (ab154907)Immunofluorescence analysis of ice-cold MeOH HepG2 (human liver hepatocellular carcinoma cell line) whole cell labeling RPL4 at cytoplasm with ab154907 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL4 antibody (ab154907)Immunohistochemical analysis of paraffin-embedded mouse colon tissue staining RPL4 at cytosol with ab154907 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154907 は 1 報の論文で使用されています。
- Ouyang H et al. Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development. Front Physiol 8:281 (2017). WB . PubMed: 28533755