Anti-RPL29 抗体 (ab67196)
Key features and details
- Mouse polyclonal to RPL29
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL29 antibody
RPL29 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to RPL29 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human RPL29 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Human pancreas tissue lysate and 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 18 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 18 kDa). |
ターゲット情報
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配列類似性
Belongs to the ribosomal protein L29e family. - Information by UniProt
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参照データベース
- Entrez Gene: 6159 Human
- Omim: 601832 Human
- SwissProt: P47914 Human
- Unigene: 425125 Human
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別名
- 60S ribosomal protein L29 antibody
- Cell surface heparin binding protein HIP antibody
- Cell surface heparin-binding protein HIP antibody
see all
画像
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Western blot - Anti-RPL29 antibody (ab67196)Anti-RPL29 antibody (ab67196) at 1/500 dilution + human pancreas tissue lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Western blot - Anti-RPL29 antibody (ab67196)All lanes : Anti-RPL29 antibody (ab67196) at 1/500 dilution
Lane 1 : RPL29 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab67196 は 1 報の論文で使用されています。
- Zhu G et al. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. Front Oncol 11:681366 (2021). PubMed: 34136404
