AB254927

Anti-RPL28 抗体

Name: Anti-RPL28 抗体 (ab254927) | Abcam
Brand: Abcam Limited
SKU: AB254927
Price: 93000 JPY
Availability: InStock

Anti-RPL28 antibody

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(2 Publications)

Rabbit Polyclonal RPL28 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RPL28 aa 50 to C-terminus.

別名を表示する

Large ribosomal subunit protein eL28, 60S ribosomal protein L28, RPL28

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Paraffin-embedded human Fallopian tube tissue stained for RPL28 using ab254927 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RPL28 antibody (AB254927)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL28 antibody (AB254927)

PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling RPL28 using ab254927 at 4µg/ml (green) in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Paraffin-embedded human cerebral cortex tissue stained for RPL28 using ab254927 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Paraffin-embedded human gastrointestinal tissue stained for RPL28 using ab254927 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Paraffin-embedded human lymphiod tissue stained for RPL28 using ab254927 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-RPL28 antibody (AB254927)

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Western blot - Anti-RPL28 antibody (ab254927)

Lanes 2 - 4:

Western blot - Anti-RPL28 antibody (ab254927) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

U2OS cells transfected with the control siRNA

Lane 3:

U2OS cells transfected with the siRNA probe 1

Lane 4:

U2OS cells transfected with the siRNA probe 2

Predicted band size: 16 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human RPL28 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46779

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ribosomal protein L28 (RPL28) also known as 60S ribosomal protein L28 plays a mechanical role in the formation of ribosomes which are essential for protein synthesis. RPL28 forms part of the large 60S subunit of the ribosome. This protein has a molecular mass of approximately 15-17 kDa and is expressed in various tissues due to its fundamental role in cellular functions. The expression of RPL28 occurs widely but can change in response to cellular activity related to protein production needs.

Biological function summary

RPL28 participates in the assembly and function of ribosomes within the cell. It acts as a structural component of the ribosomal large subunit and therefore helps in the synthesis of proteins by translating mRNA. As part of the ribosomal complex RPL28 ensures the proper alignment of rRNA and tRNA during protein assembly. Its function is integral to the ribosome’s role in facilitating correct translation steps needed for cellular protein production.

Pathways

RPL28 has important roles in the protein synthesis (translation) and ribosome biogenesis pathways. It interacts with other ribosomal proteins and factors involved in these pathways. RPL28 coordinates with proteins such as RPL5 and RPL11 which are also involved in the assembly of the large ribosomal subunit and ensure effective translation and synthesis of proteins required by the cell.

Mutations or alterations in RPL28 expression have connections to disorders of ribosome dysfunction known as ribosomopathies. Such conditions may include Diamond-Blackfan Anemia where ribosomal biogenesis is impaired possibly involving other proteins like RPL5. Deficiencies in RPL28 or associated proteins can lead to abnormal ribosome assembly affecting protein synthesis and contributing to disease symptoms.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 32669547).

See full target information RPL28

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedical research (Tokyo, Japan) 46:93-99 PubMed40436766

2025

The predictive value of MISP for the postoperative outcomes of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Jin,Lei Xin,Hao Wang,Yunpeng Lin,Yan Zhou

PLoS genetics 19:e1010797 PubMed37307272

2023

Retrotransposon LINE-1 bodies in the cytoplasm of piRNA-deficient mouse spermatocytes: Ribonucleoproteins overcoming the integrated stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara De Luca,Anuj Gupta,Alex Bortvin

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL28 抗体

Anti-RPL28 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Immunocytochemistry/ Immunofluorescence - Anti-RPL28 antibody (AB254927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL28 antibody (AB254927)

Western blot - Anti-RPL28 antibody (AB254927)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise