AB59567

Anti-RPL26 + RPL26L1 抗体

Name: Anti-RPL26 + RPL26L1 抗体 (ab59567) | Abcam
Brand: Abcam Limited
SKU: AB59567
Price: 49000 JPY
Availability: InStock
Rating: 3 (3 reviews)

Anti-RPL26 + RPL26L1 antibody

(3 Reviews)

(38 Publications)

Anti-RPL26 + RPL26L1 antibody (ab59567) is a rabbit polyclonal antibody detecting RPL26 in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 30 publications
- Trusted since 2007

別名を表示する

Large ribosomal subunit protein uL24, 60S ribosomal protein L26, RPL26

3 Images

Lab

Western blot - Anti-RPL26 + RPL26L1 antibody (AB59567)

This antibody cross-reacts with RPL26 and RPL26L1.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used a a GAPDH loading control.

All lanes:

Western blot - Anti-RPL26 + RPL26L1 antibody (ab59567) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a human RL26 expression vector containing a His tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a human RL26L1 expression vector containing a His tag, whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with an empty vector containing a His tag, whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 17-23 kDa

false

Exposure time: 15s

Immunocytochemistry/ Immunofluorescence - Anti-RPL26 + RPL26L1 antibody (AB59567)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL26 + RPL26L1 antibody (AB59567)

Cytoplasmic localization of RPL26 in HeLa cells stained with ab59567 at a 5μg/mL concentration (A), and DAPI (B). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugated.

Unknown

Western blot - Anti-RPL26 + RPL26L1 antibody (AB59567)

All lanes:

Western blot - Anti-RPL26 + RPL26L1 antibody (ab59567) at 0.25 µg/mL

Lane 1:

293T

Lane 2:

HeLa

Lane 3:

U2OS

Lane 4:

MCF7

Lane 5:

K562

Lane 6:

A431

Lane 7:

Jurkat

Lane 8:

HepG2

Lane 9:

NIH3T3

Lane 10:

PC12

Lane 11:

RAT1

Secondary

All lanes:

Goat Anti-rabbit IgG, Peroxidase conjugate

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human RPL26 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P61254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-11 µg/mL", "WB-species-notes": "Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-RPL26 + RPL26L1 antibody (ab59567) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of RPL26?
Anti-RPL26 + RPL26L1 (ab59567) specifically detects a band for RPL26 (UniProt: P61254) at a molecular weight of 17kDa.

Trusted by the scientific community
Anti-RPL26 + RPL26L1 (ab59567) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPL26 also known as 60S ribosomal protein L26 or RPL26L1 is a component of the ribosome specifically part of the 60S large ribosomal subunit. It plays a role in ribosome biogenesis and protein synthesis. The molecular mass of RPL26 is approximately 17 kDa. It is expressed in a variety of tissues reflecting its fundamental role in protein production across different cell types. Expression levels might vary based on cellular activity and demands.

Biological function summary

RPL26 participates in the assembly and stabilization of ribosomal RNA and ribosome structure. It contributes to the formation of the ribosome complex important for translating mRNA into functional proteins. RPL26 interacts with multiple ribosomal proteins and other factors within the nucleolus to facilitate efficient ribosome production positioning it as an essential player in cellular growth and proliferation.

Pathways

RPL26 plays an integral role in the process of translation part of the broader cell cycle control and growth regulation pathways. It is associated with the p53 signaling pathway which involves regulation of the cell cycle and response to DNA damage through interactions with proteins such as MDM2. These interactions highlight its involvement in cellular responses to stress and damage linking ribosomal function with regulation of cell survival mechanisms.

Mutations or dysregulation of RPL26 have been connected to certain cancers with studies indicating its role in tumor progression in conditions like breast cancer. Furthermore alterations in RPL26 expression or function may contribute to ribosomopathies disorders that involve defects in ribosome function leading to conditions like Diamond-Blackfan Anemia. In these contexts RPL26 interacts with tumor suppressor proteins like p53 highlighting its influence on cell growth and apoptosis in disease states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the large ribosomal subunit (PubMed : 23636399, PubMed : 26100019, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 23636399, PubMed : 26100019, PubMed : 32669547).

See full target information RPL26

追加のターゲット

RPL26L1

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文献 (38)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx4511 PubMed40938992

2025

The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Vikhe Patil,Nil Campamà Sanz,Kylie Hin-Man Mak,Wei Yang,Karl Annusver,Jasson Makkar,David Grommisch,Li Lei,Priyanka Sharma,Ryan R Driskell,Maria Kasper,Maria Genander

Nature communications 16:3912 PubMed40280917

2025

UFC1 reveals the multifactorial and plastic nature of oxyanion holes in E2 conjugating enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Manoj Kumar,Sayanika Banerjee,Einav Cohen-Kfir,Marissa Basia Mitelberg,Suryakant Tiwari,Michail N Isupov,Moshe Dessau,Reuven Wiener

Frontiers in molecular neuroscience 18:1427036 PubMed40051914

2025

Astrocyte-secreted factors modulate synaptic protein synthesis as revealed by puromycin labeling of isolated synaptosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida de la Cruz-Gambra,Jimena Baleriola

Autophagy 21:996-1018 PubMed39842454

2025

The Epstein-Barr virus deubiquitinase BPLF1 regulates stress-induced ribosome UFMylation and reticulophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangnan Liu,Noemi Nagy,Carlos Ayala-Torres,Solenne Bleuse,Francisco Aguilar-Alonso,Ola Larsson,Maria G Masucci

Nature 627:445-452 PubMed38383785

2024

UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A DaRosa,Ivan Penchev,Samantha C Gumbin,Francesco Scavone,Magda Wąchalska,Joao A Paulo,Alban Ordureau,Joshua J Peter,Yogesh Kulathu,J Wade Harper,Thomas Becker,Roland Beckmann,Ron R Kopito

Science advances 9:eadh3635 PubMed37595036

2023

Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Ishimura,Sota Ito,Gaoxin Mao,Satoko Komatsu-Hirota,Toshifumi Inada,Nobuo N Noda,Masaaki Komatsu

Nucleic acids research 51:3375-3390 PubMed36881761

2023

Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shir Mandelboum,Melisa Herrero,Andrea Atzmon,Marcelo Ehrlich,Orna Elroy-Stein

Nature communications 13:7857 PubMed36543799

2022

The UFM1 system regulates ER-phagy through the ufmylation of CYB5R3.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Ishimura,Afnan H El-Gowily,Daisuke Noshiro,Satoko Komatsu-Hirota,Yasuko Ono,Mayumi Shindo,Tomohisa Hatta,Manabu Abe,Takefumi Uemura,Hyeon-Cheol Lee-Okada,Tarek M Mohamed,Takehiko Yokomizo,Takashi Ueno,Kenji Sakimura,Tohru Natsume,Hiroyuki Sorimachi,Toshifumi Inada,Satoshi Waguri,Nobuo N Noda,Masaaki Komatsu

EMBO reports 24:e53801 PubMed36472244

2022

Piwil2 (Mili) sustains neurogenesis and prevents cellular senescence in the postnatal hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Gasperini,Kiril Tuntevski,Silvia Beatini,Roberta Pelizzoli,Amanda Lo Van,Damiano Mangoni,Rosa M Cossu,Giovanni Pascarella,Paolo Bianchini,Pascal Bielefeld,Margherita Scarpato,Meritxell Pons-Espinal,Remo Sanges,Alberto Diaspro,Carlos P Fitzsimons,Piero Carninci,Stefano Gustincich,Davide De Pietri Tonelli

Nucleic acids research 50:10756-10771 PubMed36165847

2022

Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Ambrosini,Eliana Destefanis,Eyemen Kheir,Francesca Broso,Federica Alessandrini,Sara Longhi,Nicolò Battisti,Isabella Pesce,Erik Dassi,Gianluca Petris,Anna Cereseto,Alessandro Quattrone

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL26 + RPL26L1 抗体

Anti-RPL26 + RPL26L1 antibody

Western blot - Anti-RPL26 + RPL26L1 antibody (AB59567)

Immunocytochemistry/ Immunofluorescence - Anti-RPL26 + RPL26L1 antibody (AB59567)

Western blot - Anti-RPL26 + RPL26L1 antibody (AB59567)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (38)

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise