Anti-RPL26 + RPL26L1 抗体 (ab59567)
Key features and details
- Rabbit polyclonal to RPL26 + RPL26L1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL26 + RPL26L1 antibody -
製品の詳細
Rabbit polyclonal to RPL26 + RPL26L1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Xenopus laevis, Cynomolgus monkey
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免疫原
Synthetic peptide corresponding to Human RPL26 aa 1-17 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:MKFNPFVTSDRSKNRKR
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59567の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 - 10 µg/ml.
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| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide. |
| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Staining of the ribosomal protein L26 band in immunoblotting is specifically inhibited by the immunizing peptide. |
画像
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Western blot - Anti-RPL26 + RPL26L1 antibody (ab59567)All lanes : Anti-RPL26 + RPL26L1 antibody (ab59567) at 1/1000 dilution
Lane 1 : HEK-293T cells transfected with a human RL26 expression vector containing a His tag, whole cell lysate
Lane 2 : HEK-293T cells transfected with a human RL26L1 expression vector containing a His tag, whole cell lysate
Lane 3 : HEK-293T cells transfected with an empty vector containing a His tag, whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 17 kDa
Observed band size: 17-23 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis antibody cross-reacts with RPL26 and RPL26L1.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
ab181602 was used a a GAPDH loading control.
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Immunocytochemistry/ Immunofluorescence - Anti-RPL26 + RPL26L1 antibody (ab59567)Cytoplasmic localization of RPL26 in HeLa cells stained with ab59567 at a 5µg/mL concentration (A), and DAPI (B). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugated.
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Western blot - Anti-RPL26 + RPL26L1 antibody (ab59567)All lanes : Anti-RPL26 + RPL26L1 antibody (ab59567) at 0.25 µg/ml
Lane 1 : 293T
Lane 2 : HeLa
Lane 3 : U2OS
Lane 4 : MCF7
Lane 5 : K562
Lane 6 : A431
Lane 7 : Jurkat
Lane 8 : HepG2
Lane 9 : NIH3T3
Lane 10 : PC12
Lane 11 : RAT1
Secondary
All lanes : Goat Anti-rabbit IgG, Peroxidase conjugate
Predicted band size: 17 kDa
Observed band size: 17 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (31)
ab59567 は 31 報の論文で使用されています。
- Ishimura R et al. Mechanistic insights into the roles of the UFM1 E3 ligase complex in ufmylation and ribosome-associated protein quality control. Sci Adv 9:eadh3635 (2023). PubMed: 37595036
- Gasperini C et al. Piwil2 (Mili) sustains neurogenesis and prevents cellular senescence in the postnatal hippocampus. EMBO Rep 24:e53801 (2023). PubMed: 36472244
- Mandelboum S et al. Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes. Nucleic Acids Res 51:3375-3390 (2023). PubMed: 36881761
- Millrine D et al. Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation. Cell Rep 40:111168 (2022). PubMed: 35926457
- de Sousa E et al. VDAC1 regulates neuronal cell loss after retinal trauma injury by a mitochondria-independent pathway. Cell Death Dis 13:393 (2022). PubMed: 35449127