AB241087

Anti-RPL23 抗体

Name: Anti-RPL23 抗体 (ab241087) | Abcam
Brand: Abcam Limited
SKU: AB241087
Price: 75000 JPY
Availability: InStock

Anti-RPL23 antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal RPL23 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RPL23 aa 50-100.

別名を表示する

Large ribosomal subunit protein uL14, 60S ribosomal protein L17, 60S ribosomal protein L23, RPL23

2 Images

Supplier Data

Immunoprecipitation - Anti-RPL23 antibody (AB241087)

RPL23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241087 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241087 at 1 μg/ml.

Lane 1 : ab241087 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RPL23 antibody (ab241087)

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-RPL23 antibody (AB241087)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RPL23 antibody (ab241087) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 15 kDa

true

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

WB, IP

applications

免疫原

Synthetic Peptide within Human RPL23 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P62829

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

精製に関する特記事項

Antibody was affinity purified using an epitope specific to RPL23 immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPL23 also known as ribosomal protein L23 is an essential component of the large 60S ribosomal subunit with a molecular mass of approximately 15 kDa. This protein is highly expressed in eukaryotic cells particularly in tissues with high rates of protein synthesis. RPL23 interacts directly with rRNA to facilitate its structural stability and function playing an important role in the assembly of the ribosome and the translation of mRNA into proteins.

Biological function summary

RPL23 contributes to the synthesis of proteins by forming part of the ribosomal complex. Ribosomes are composed of two subunits 40S and 60S in eukaryotes with RPL23 being integral to the larger subunit. The presence of RPL23 ensures that the ribosomes effectively translate the genetic code carried by mRNA into a polypeptide chain. This protein is essential in maintaining the fidelity and efficiency of translation processes within cells determining how cells grow and respond to various stimuli.

Pathways

RPL23 plays a significant role in the translation initiation pathway and the protein synthesis pathway which are important for cell growth and replication. Within these pathways RPL23 associates with other ribosomal proteins such as RPL5 and RPL11 which together modulate the formation of the ribosomal structure and oversee the translation process. The function of RPL23 in these pathways ensures that cellular resources are properly managed and directed towards productive protein synthesis.

Mutations or dysregulation of RPL23 have connections to cancer and Diamond-Blackfan anemia. The alteration in RPL23 can disrupt normal ribosome function leading to impaired protein synthesis and contributing to the uncontrolled cell growth seen in cancers. This protein also interacts with tumor suppressor proteins like TP53 linking its activity to mechanisms that control cell cycle and apoptosis. Furthermore deficiencies in RPL23 function may lead to insufficient production of red blood cells evident in Diamond-Blackfan anemia highlighting a relationship with genes responsible for balanced erythropoiesis.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.

See full target information RPL23

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of medical genetics. Part A 191:2757-2767 PubMed37596828

2023

Novel association of Dandy-Walker malformation with CAPN15 variants expands the phenotype of oculogastrointestinal neurodevelopmental syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

M Makenzie Beaman,Lucia Guidugli,Monia Hammer,Chelsea Barrows,Anne Gregor,Sangmoon Lee,Kristen L Deak,Marie T McDonald,Courtney Jensen,Maha S Zaki,Amira T Masri,Charlotte A Hobbs,Joseph G Gleeson,Jennifer L Cohen

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPL23 抗体

Anti-RPL23 antibody

Immunoprecipitation - Anti-RPL23 antibody (AB241087)

Western blot - Anti-RPL23 antibody (AB241087)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise