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AB254898

Anti-RPL18 抗体

Anti-RPL18 antibody

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(1 Publication)

Rabbit Polyclonal RPL18 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RPL18 aa 50 to C-terminus.

別名を表示する

Large ribosomal subunit protein eL18, 60S ribosomal protein L18, RPL18

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RPL18 antibody (AB254898)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RPL18 antibody (AB254898)

PFA fixed, Triton X-100 permeabilized HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling RPL18 using ab254898 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL18 antibody (AB254898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL18 antibody (AB254898)

Paraffin-embedded human pancreas tissue stained for RPL18 using ab254898 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-RPL18 antibody (AB254898)
  • WB

Supplier Data

Western blot - Anti-RPL18 antibody (AB254898)

Lane 1:

Western blot - Anti-RPL18 antibody (ab254898)

Lanes 2 - 3:

Western blot - Anti-RPL18 antibody (ab254898) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 3:

U-251MG sp whole cell lysate

Predicted band size: 22 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human RPL18 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q07020

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RPL18 also known as Ribosomal Protein L18 is a component of the 60S large ribosomal subunit. It possesses a molecular weight of approximately 22 kDa. RPL18 is highly expressed in various tissues including the liver brain and pancreas. The protein plays an important role in protein synthesis by aiding in the assembly and function of ribosomes essential cellular machinery for translating mRNA into polypeptides.
Biological function summary

RPL18 functions as part of the ribosomal complex facilitating mRNA translation into proteins. This protein is integral to the structure of the ribosome which serves as the platform for accurate peptide bond formation between amino acids. RPL18 like other ribosomal proteins contributes to the stability and efficiency of the ribosome's function during the translation process. Its presence ensures the proper alignment and interaction of ribosomal RNA and other ribosomal proteins.

Pathways

RPL18 is involved in key processes like the protein synthesis pathway and the ribosomal biogenesis pathway. In these pathways RPL18 interacts closely with other ribosomal proteins such as RPL5 and RPL11 which together form the core of the ribosomal assembly machinery. It also plays a role in the regulation of p53 through its interaction with these and other ribosomal proteins that influence cellular stress responses and cell cycle arrest.

Alterations in RPL18 levels or function can be linked to cancer and Diamond-Blackfan anemia. In cancer biology changes in ribosomal proteins including RPL18 may lead to disruptions in the normal patterns of protein synthesis contributing to tumorigenesis. In Diamond-Blackfan anemia a disorder characterized by bone marrow failure mutations in ribosomal proteins like RPL18 can impair ribosome function and affect hematopoiesis. RPL18's association with these conditions often involves interactions with key proteins such as p53 highlighting its broader impact on disease mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the large ribosomal subunit (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed : 12962325, PubMed : 23636399, PubMed : 25901680, PubMed : 25957688, PubMed : 32669547).
See full target information RPL18
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 40:134 PubMed33858489

2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junshuang Zhao,Yang Jiang,Haiying Zhang,Jinpeng Zhou,Lian Chen,Hao Li,Jinkun Xu,Guoqing Zhang,Zhitao Jing
View all publications
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