Anti-RPL12 抗体 (ab157130)
Key features and details
- Rabbit polyclonal to RPL12
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPL12 antibody
RPL12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL12 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to a region between residues 115-165 of Human RPL12 (NP_000967.1).
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ポジティブ･コントロール
- Jurkat, HeLa, 293T and mouse NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157130の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 18 kDa.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 18 kDa. |
ターゲット情報
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機能
Binds directly to 26S ribosomal RNA. -
配列類似性
Belongs to the ribosomal protein L11P family. - Information by UniProt
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参照データベース
- Entrez Gene: 6136 Human
- Entrez Gene: 269261 Mouse
- Entrez Gene: 499782 Rat
- Entrez Gene: 685320 Rat
- Omim: 180475 Human
- SwissProt: P30050 Human
- SwissProt: P35979 Mouse
- SwissProt: P23358 Rat
see all -
別名
- 60S ribosomal protein L12 antibody
- L12 antibody
- Ribosomal protein L12 antibody
see all
画像
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Western blot - Anti-RPL12 antibody (ab157130)All lanes : Anti-RPL12 antibody (ab157130) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab157130 は 3 報の論文で使用されています。
- Sharma J et al. A small molecule that induces translational readthrough of CFTR nonsense mutations by eRF1 depletion. Nat Commun 12:4358 (2021). PubMed: 34272367
- Oliver KE et al. Slowing ribosome velocity restores folding and function of mutant CFTR. J Clin Invest 129:5236-5253 (2019). PubMed: 31657788
- Veit G et al. Ribosomal Stalk Protein Silencing Partially Corrects the ?F508-CFTR Functional Expression Defect. PLoS Biol 14:e1002462 (2016). WB ; Human . PubMed: 27168400