Anti-RPL11 抗体 (ab79352)
Key features and details
- Rabbit polyclonal to RPL11
- Suitable for: IHC-P, WB, IP
- Reacts with: Human, Zebrafish
- Isotype: IgG
製品の概要
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製品名
Anti-RPL11 antibody
RPL11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPL11 -
由来種
Rabbit -
特異性
From December 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human, Zebrafish
交差が予測される動物種: Mouse, Rat, Cow, Cat, Orangutan
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免疫原
Synthetic peptide corresponding to Human RPL11 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88221) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa).
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| IP |
Use at an assay dependent concentration.
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| 特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). |
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IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds to 5S ribosomal RNA (By similarity). Required for rRNA maturation and formation of the 60S ribosomal subunits. Promotes nucleolar location of PML. -
関連疾患
Defects in RPL11 are the cause of Diamond-Blackfan anemia type 7 (DBA7) [MIM:612562]. DBA7 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. -
配列類似性
Belongs to the ribosomal protein L5P family. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 6135 Human
- Entrez Gene: 67025 Mouse
- Entrez Gene: 362631 Rat
- Entrez Gene: 415229 Zebrafish
- Omim: 604175 Human
- SwissProt: P62913 Human
- SwissProt: Q9CXW4 Mouse
- SwissProt: P62914 Rat
see all -
別名
- 60S ribosomal protein L11 antibody
- Cell growth inhibiting protein 34 antibody
- Cell growth inhibiting protein34 antibody
see all
画像
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Western blot - Anti-RPL11 antibody (ab79352)All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 7 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 30 kDa, 45 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
RPL11 contains a number of potential phosphorylation and acetylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunoprecipitation - Anti-RPL11 antibody (ab79352)RPL11 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to RPL11 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79352.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 22kDa: RPL11 -
- Anti-RPL11 antibody (ab79352)IHC image of ab79352 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79352, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-RPL11 antibody (ab79352)All lanes : Anti-RPL11 antibody (ab79352) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : Human hepatocellular carcinoma cell line at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab79352 は 23 報の論文で使用されています。
- Gan Y et al. UTP11 deficiency suppresses cancer development via nucleolar stress and ferroptosis. Redox Biol 62:102705 (2023). PubMed: 37087976
- Takada Y et al. Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development. Sci Adv 9:eadg6532 (2023). PubMed: 38000026
- Dörner K et al. Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. Nucleic Acids Res 50:2872-2888 (2022). PubMed: 35150276
- Stoneley M et al. Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. Mol Cell 82:1557-1572.e7 (2022). PubMed: 35180429
- Park J et al. MicroRNA-101-3p Suppresses Cancer Cell Growth by Inhibiting the USP47-Induced Deubiquitination of RPL11. Cancers (Basel) 14:N/A (2022). PubMed: 35205710