AB182150

Anti-RPB3 抗体 [EPR13294(B)]

Anti-RPB3 antibody [EPR13294(B)]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal RPB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

別名を表示する

A-152E5.7, POLR2C, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31, RPB33

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling RPB3 with ab182150 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

All lanes:

Western blot - Anti-RPB3 antibody [EPR13294(B)] (ab182150) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 31 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13294(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.

See full target information POLR2C

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed39791720

2025

Induction of SUSD2 by STAT3 Activation Is Associated with Tumor Recurrence in HER2-Positive Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yisun Jeong,Hyungjoo Kim,Daeun You,Soo Youn Cho,Sun Young Yoon,Seok Won Kim,Seok Jin Nam,Jeong Eon Lee,Sangmin Kim

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 26:183-193 PubMed35477546

2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ding,Quanhua Pan,Long Qian,Chuanxian Hu

Molecular cell 81:4413-4424.e5 PubMed34480849

2021

SPT5 stabilization of promoter-proximal RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Aoi,Yoh-Hei Takahashi,Avani P Shah,Marta Iwanaszko,Emily J Rendleman,Nabiha H Khan,Byoung-Kyu Cho,Young Ah Goo,Sheetal Ganesan,Neil L Kelleher,Ali Shilatifard

eLife 10: PubMed34085923

2021

Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Duncan-Lewis,Ella Hartenian,Valeria King,Britt A Glaunsinger

eLife 8: PubMed31385803

2019

Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua E Mayfield,Seema Irani,Edwin E Escobar,Zhao Zhang,Nathaniel T Burkholder,Michelle R Robinson,M Rachel Mehaffey,Sarah N Sipe,Wanjie Yang,Nicholas A Prescott,Karan R Kathuria,Zhijie Liu,Jennifer S Brodbelt,Yan Zhang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RPB3 抗体 [EPR13294(B)]

Anti-RPB3 antibody [EPR13294(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

Western blot - Anti-RPB3 antibody [EPR13294(B)] (AB182150)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (5)

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Abcam product promise